BBa_J69021
type: ComponentDefinition
persistent identity: BBa J69021
display id: BBa_J69021
version: 1
was generated by: iGEM to SBOL conversion
description: Lux Auto-inducible Device 2
created: 2008-09-22T11:00:00Z
modified: 2015-05-08T01:08:18Z
mutable provenance: NA
owned by: james
owned by: myers
mutable description: NA
discontinued: false
dominant: false
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: NA
star: false
creator: James Brown
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J28031
component: BBa_J69524
sequence annotation: BBa_J69524
sequence annotation: BBa_J28031
sequence: https://synbiohub.org/public/igem/BBa_J69021_sequence/1

BBa_J28031
type: Component
persistent identity: component2224071
display id: component2224071
version: 1
top level: BBa_J69021
definition: BBa_J28031
access: public

BBa_J69524
type: Component
persistent identity: component2224070
display id: component2224070
version: 1
top level: BBa_J69021
definition: BBa_J69524
access: public

BBa_J69524
type: SequenceAnnotation
persistent identity: annotation2224070
display id: annotation2224070
version: 1
top level: BBa_J69021
location: https://synbiohub.org/public/igem/BBa_J69021/annotation2224070/range2224070/1
component: BBa_J69524

https://synbiohub.org/public/igem/BBa_J69021/annotation2224070/range2224070/1
type: Range
persistent identity: range2224070
display id: range2224070
version: 1
top level: BBa_J69021
direction: direction/reverse
start: 1
end: 940
orientation: inline

BBa_J28031
type: SequenceAnnotation
persistent identity: annotation2224071
display id: annotation2224071
version: 1
top level: BBa_J69021
location: https://synbiohub.org/public/igem/BBa_J69021/annotation2224071/range2224071/1
component: BBa_J28031

https://synbiohub.org/public/igem/BBa_J69021/annotation2224071/range2224071/1
type: Range
persistent identity: range2224071
display id: range2224071
version: 1
top level: BBa_J69021
direction: direction/reverse
start: 949
end: 2629
orientation: inline

BBa_J69524
type: ComponentDefinition
persistent identity: BBa J69524
display id: BBa_J69524
version: 1
was generated by: iGEM to SBOL conversion
description: tetR-repressible lacI generator
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:08:19Z
mutable provenance: NA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: NA
discontinued: false
dominant: false
experience: experience/None
group u list: _101_
m user id: 0
owner id: 740
owning group id: 101
sample status: In stock
status: status/Available
bookmark: false
mutable notes: NA
star: false
creator: James Brown
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_R0040
component: BBa_E0030
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_J69524_sequence/1

BBa_R0040
type: Component
persistent identity: component2166316
display id: component2166316
version: 1
top level: BBa_J69524
definition: p(tetR)
access: public

BBa_E0030
type: Component
persistent identity: component2166323
display id: component2166323
version: 1
top level: BBa_J69524
definition: eyfp
access: public

BBa_B0034
type: Component
persistent identity: component2166322
display id: component2166322
version: 1
top level: BBa_J69524
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2166330
display id: component2166330
version: 1
top level: BBa_J69524
definition: BBa_B0015
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2166316
display id: annotation2166316
version: 1
top level: BBa_J69524
location: https://synbiohub.org/public/igem/BBa_J69524/annotation2166316/range2166316/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J69524/annotation2166316/range2166316/1
type: Range
persistent identity: range2166316
display id: range2166316
version: 1
top level: BBa_J69524
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2166322
display id: annotation2166322
version: 1
top level: BBa_J69524
location: https://synbiohub.org/public/igem/BBa_J69524/annotation2166322/range2166322/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J69524/annotation2166322/range2166322/1
type: Range
persistent identity: range2166322
display id: range2166322
version: 1
top level: BBa_J69524
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation2166323
display id: annotation2166323
version: 1
top level: BBa_J69524
location: https://synbiohub.org/public/igem/BBa_J69524/annotation2166323/range2166323/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J69524/annotation2166323/range2166323/1
type: Range
persistent identity: range2166323
display id: range2166323
version: 1
top level: BBa_J69524
direction: direction/reverse
start: 81
end: 803
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2166330
display id: annotation2166330
version: 1
top level: BBa_J69524
location: https://synbiohub.org/public/igem/BBa_J69524/annotation2166330/range2166330/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_J69524/annotation2166330/range2166330/1
type: Range
persistent identity: range2166330
display id: range2166330
version: 1
top level: BBa_J69524
direction: direction/reverse
start: 812
end: 940
orientation: inline

BBa_J28031
type: ComponentDefinition
persistent identity: BBa J28031
display id: BBa_J28031
version: 1
was generated by: iGEM to SBOL conversion
description: lux autoinducer
created: 2006-10-23T11:00:00Z
modified: 2015-08-31T04:08:44Z
mutable provenance: soon
owned by: james
owned by: myers
mutable description: soon
discontinued: false
dominant: false
experience: experience/None
group u list: _101_
m user id: 0
owner id: 1072
owning group id: 57
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: soon
star: true
creator: Cambridge iGEM 2006 Team
type: DnaRegion
role: SO:0000804
role: partType/Signalling
sequence annotation: BBa_B0015
sequence annotation: BBa_J28031
sequence annotation: SphI restriction site
sequence annotation: BBa_C0062 (luxR), codons optimised for E. coli
sequence annotation: BBa_B0034
sequence annotation: BamHI restriction site
sequence annotation: BBa_R0011 (lac, lambda cI hybrid)
sequence annotation: BspHI restriction site
sequence annotation: BBa_R0062 (lux pR)
sequence annotation: SalI restriction site
sequence annotation: BBa_C0061 (luxI), codons optimised for E. coli
sequence: https://synbiohub.org/public/igem/BBa_J28031_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation1904926
display id: annotation1904926
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904926/range1904926/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J28031/annotation1904926/range1904926/1
type: Range
persistent identity: range1904926
display id: range1904926
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_J28031
type: SequenceAnnotation
persistent identity: annotation1904937
display id: annotation1904937
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904937/range1904937/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J28031/annotation1904937/range1904937/1
type: Range
persistent identity: range1904937
display id: range1904937
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 1
end: 1681
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation1904927
display id: annotation1904927
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904927/range1904927/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J28031/annotation1904927/range1904927/1
type: Range
persistent identity: range1904927
display id: range1904927
version: 1
top level: BBa_J28031
direction: direction/forward
start: 131
end: 136
orientation: inline

BBa_C0062 (luxR), codons optimised for E. coli
type: SequenceAnnotation
persistent identity: annotation1904928
display id: annotation1904928
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904928/range1904928/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J28031/annotation1904928/range1904928/1
type: Range
persistent identity: range1904928
display id: range1904928
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 138
end: 893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1904929
display id: annotation1904929
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904929/range1904929/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J28031/annotation1904929/range1904929/1
type: Range
persistent identity: range1904929
display id: range1904929
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 900
end: 911
orientation: inline

BamHI restriction site
type: SequenceAnnotation
persistent identity: annotation1904930
display id: annotation1904930
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904930/range1904930/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J28031/annotation1904930/range1904930/1
type: Range
persistent identity: range1904930
display id: range1904930
version: 1
top level: BBa_J28031
direction: direction/forward
start: 913
end: 918
orientation: inline

BBa_R0011 (lac, lambda cI hybrid)
type: SequenceAnnotation
persistent identity: annotation1904931
display id: annotation1904931
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904931/range1904931/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J28031/annotation1904931/range1904931/1
type: Range
persistent identity: range1904931
display id: range1904931
version: 1
top level: BBa_J28031
direction: direction/reverse
start: 920
end: 974
orientation: inline

BspHI restriction site
type: SequenceAnnotation
persistent identity: annotation1904932
display id: annotation1904932
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904932/range1904932/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J28031/annotation1904932/range1904932/1
type: Range
persistent identity: range1904932
display id: range1904932
version: 1
top level: BBa_J28031
direction: direction/forward
start: 976
end: 981
orientation: inline

BBa_R0062 (lux pR)
type: SequenceAnnotation
persistent identity: annotation1904933
display id: annotation1904933
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904933/range1904933/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J28031/annotation1904933/range1904933/1
type: Range
persistent identity: range1904933
display id: range1904933
version: 1
top level: BBa_J28031
direction: direction/forward
start: 983
end: 1037
orientation: inline

SalI restriction site
type: SequenceAnnotation
persistent identity: annotation1904934
display id: annotation1904934
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904934/range1904934/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J28031/annotation1904934/range1904934/1
type: Range
persistent identity: range1904934
display id: range1904934
version: 1
top level: BBa_J28031
direction: direction/forward
start: 1039
end: 1044
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1904935
display id: annotation1904935
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904935/range1904935/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J28031/annotation1904935/range1904935/1
type: Range
persistent identity: range1904935
display id: range1904935
version: 1
top level: BBa_J28031
direction: direction/forward
start: 1046
end: 1057
orientation: inline

BBa_C0061 (luxI), codons optimised for E. coli
type: SequenceAnnotation
persistent identity: annotation1904936
display id: annotation1904936
version: 1
top level: BBa_J28031
location: https://synbiohub.org/public/igem/BBa_J28031/annotation1904936/range1904936/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J28031/annotation1904936/range1904936/1
type: Range
persistent identity: range1904936
display id: range1904936
version: 1
top level: BBa_J28031
direction: direction/forward
start: 1064
end: 1681
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_J69021_sequence/1
type: Sequence
persistent identity: BBa J69021 sequence
display id: BBa_J69021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgggcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttggtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgcccatgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatcaaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggagtgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggtttttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggttagagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtagttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaaccattttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggtgtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatgtcgacgaaagagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J69524_sequence/1
type: Sequence
persistent identity: BBa J69524 sequence
display id: BBa_J69524_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J28031_sequence/1
type: Sequence
persistent identity: BBa J28031 sequence
display id: BBa_J28031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgggcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttggtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgcccatgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatcaaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggagtgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggtttttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggttagagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtagttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaaccattttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggtgtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcacaatgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatgtcgacgaaagaggagaaatactagatgaccattatgatcaagaaatctgacttcctggccatcccgtccgaagaatacaaaggcatcctgtctctgcgctaccaggttttcaaacagcgtctggaatgggacctggtggttgaaaacaacctggaatctgacgagtatgataattccaacgcagaatacatctatgcgtgtgatgatactgaaaacgtttctggttgttggcgtctgctgcctaccacgggcgattatatgctgaagtccgtatttccggagctgctgggtcagcagtccgcgccgaaagacccgaacattgtagaactgtctcgcttcgcggttggcaagaacagctctaaaatcaacaactccgcaagcgaaattaccatgaaactgttcgaagctatttataaacacgcggtttcccagggtatcaccgaatacgtgaccgtcactagcaccgctatcgagcgcttcctgaaacgtatcaaggttccgtgccaccgtatcggtgacaaagaaatccacgtactgggtgataccaagagcgtcgtgctgtccatgccgattaacgagcagtttaagaaagctgtgctgaacgccgctaacgacgaaaactacgcgctggtggcgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z