LOCUS BBa_J69022 3518 bp DNA linear 22-Nov-2024 DEFINITION Lux Auto-inducible Device 3 ACCESSION BBa_J69022 VERSION BBa_J69022.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 misc_feature 1667..1698 /label=LVA CDS 1088..1666 /label=luxI misc_feature 1088..1705 /label=BBa_C0061 misc_feature 1706..1730 /label=Help:Barcodes CDS 1088..1705 /label=BBa_C0061 misc_feature 1743..1746 /label=conserved RBS 1739..1750 /label=BBa_B0034 CDS 1757..2476 /label=GFP protein CDS 1757..2476 /label=BBa_E0040 stem_loop 2496..2539 /label=stem_loop misc_feature 2485..2564 /label=BBa_B0010 terminator 2485..2564 /label=BBa_B0010 stem_loop 2580..2599 /label=T7 TE misc_feature 2606..2606 /label=stop polyA_site 2600..2613 /label=polya misc_feature 2573..2613 /label=BBa_B0012 terminator 2573..2613 /label=BBa_B0012 misc_feature 1..2613 /label=BBa_J37015 promoter 2659..2664 /label=-10 promoter 2636..2641 /label=-35 misc_feature 2646..2662 /label=OR1 misc_feature 2622..2638 /label=OR2 misc_feature 2622..2670 /label=BBa_R0051 promoter 2622..2670 /label=BBa_R0051 misc_feature 2683..2686 /label=conserved RBS 2679..2690 /label=BBa_B0034 CDS 2700..3316 /label=tetR misc_feature 3317..3350 /label=SsrA misc_feature 3357..3381 /label=Help:Barcodes CDS 2697..3356 /label=BBa_C0040 stem_loop 3401..3444 /label=stem_loop misc_feature 3390..3469 /label=BBa_B0010 terminator 3390..3469 /label=BBa_B0010 stem_loop 3485..3504 /label=T7 TE misc_feature 3511..3511 /label=stop polyA_site 3505..3518 /label=polya misc_feature 3478..3518 /label=BBa_B0012 terminator 3478..3518 /label=BBa_B0012 misc_feature 2679..3518 /label=BBa_P0440 misc_feature 2622..3518 /label=BBa_J69506 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta 1141 taaaggtatt ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt 1201 tgtagaaaat aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc 1261 ttgtgatgat actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta 1321 tatgctgaaa agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa 1381 tatagtcgaa ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc 1441 tagtgaaatt acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat 1501 tacagaatat gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt 1561 tccttgtcat cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt 1621 attgtctatg cctattaatg aacagtttaa aaaagcagtc ttaaatgctg caaacgacga 1681 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa 1741 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct 1801 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg 1861 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt 1921 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc 1981 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga 2041 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga 2101 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa 2161 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga 2221 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag 2281 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt 2341 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag 2401 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga 2461 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2521 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2581 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaacaccgt gcgtgttgac 2641 tattttacct ctggcggtga taatggttgc tactagagaa agaggagaaa tactagatgt 2701 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa 2761 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt 2821 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata 2881 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata 2941 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag 3001 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc 3061 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt 3121 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta 3181 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg 3241 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta 3301 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact 3361 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 3421 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3481 cactggctca ccttcgggtg ggcctttctg cgtttata //