##gff-version 3
##sequence-region BBa_J69022 1 3518
BBa_J69022	.	engineered_region	2622	3518	.	+	0	ID=BBa_J69506;Name=BBa_J69506
BBa_J69022	.	engineered_region	2679	3518	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_J69506
BBa_J69022	.	terminator	3478	3518	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J69022	.	stem_loop	3485	3504	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69022	.	stop_codon	3511	3511	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69022	.	polyA_site	3505	3518	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69022	.	engineered_region	3478	3518	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69022	.	terminator	3390	3469	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J69022	.	engineered_region	3390	3469	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69022	.	stem_loop	3401	3444	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69022	.	CDS	2697	3356	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J69022	.	engineered_tag	3357	3381	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J69022	.	CDS	2700	3316	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J69022	.	tag	3317	3350	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J69022	.	ribosome_entry_site	2679	2690	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J69022	.	conserved_region	2683	2686	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69022	.	promoter	2622	2670	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J69506
BBa_J69022	.	engineered_region	2622	2670	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J69022	.	non_covalent_binding_site	2646	2662	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J69022	.	non_covalent_binding_site	2622	2638	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J69022	.	promoter	2659	2664	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J69022	.	promoter	2636	2641	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J69022	.	engineered_region	1	2613	.	+	0	ID=BBa_J37015;Name=BBa_J37015
BBa_J69022	.	CDS	1757	2476	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J37015
BBa_J69022	.	CDS	1757	2476	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J69022	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_J37015
BBa_J69022	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_J69022	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J69022	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J69022	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J69022	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J69022	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J69022	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J69022	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_J69022	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J69022	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J69022	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J69022	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J69022	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J69022	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_J69022	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69022	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_J69022	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J69022	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J69022	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J69022	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J69022	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J69022	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_J69022	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J69022	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69022	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69022	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J69022	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69022	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69022	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69022	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69022	.	CDS	1088	1705	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_J37015
BBa_J69022	.	engineered_region	1088	1705	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J69022	.	tag	1667	1698	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J69022	.	engineered_tag	1706	1730	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J69022	.	CDS	1088	1666	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J69022	.	terminator	2573	2613	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J37015
BBa_J69022	.	stem_loop	2580	2599	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69022	.	stop_codon	2606	2606	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69022	.	polyA_site	2600	2613	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69022	.	engineered_region	2573	2613	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69022	.	terminator	2485	2564	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J37015
BBa_J69022	.	engineered_region	2485	2564	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69022	.	stem_loop	2496	2539	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69022	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37015
BBa_J69022	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69022	.	ribosome_entry_site	1739	1750	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37015
BBa_J69022	.	conserved_region	1743	1746	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J69022
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaa
aatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagact
tgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgt
gatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctg
aattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctc
aaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtatt
acagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggag
acaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagt
cttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagag
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagagga
gaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaa
ggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcggg
ctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggca
agattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggt
acacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagaga
atgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaa
agaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggt
gcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccg
ctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata