LOCUS       BBa_J69101              2651 bp    DNA     linear       26-Nov-2024
DEFINITION  LasAIC with terminated GFP output
ACCESSION   BBa_J69101
VERSION     BBa_J69101.1  
FEATURES             Location/Qualifiers
     stem_loop       1..129
                     /label=BBa_B0015
     misc_feature    131..136
                     /label=SphI restriction site
     CDS             138..860
                     /label=BBa_C0079 (lasR), codons optimised for E. coli
     RBS             867..878
                     /label=BBa_B0034
     misc_feature    880..885
                     /label=BamHI restriction site
     promoter        887..935
                     /label=BBa_R0051 (lamda cI)
     misc_feature    937..942
                     /label=BspHI restriction site
     promoter        944..1100
                     /label=BBa_R0079 (las pR)
     misc_feature    1102..1107
                     /label=SalI restriction site
     RBS             1109..1120
                     /label=BBa_B0034
     CDS             1127..1768
                     /label=BBa_C0078 (lasI), codons optimised for E. coli
     misc_feature    1..1768
                     /label=BBa_J28032
     misc_feature    1..1768
                     /label=BBa_J28032
     misc_feature    1781..1784
                     /label=conserved
     RBS             1777..1788
                     /label=BBa_B0034
     CDS             1795..2514
                     /label=GFP protein
     CDS             1795..2514
                     /label=BBa_E0040
     stem_loop       2534..2577
                     /label=stem_loop
     misc_feature    2523..2602
                     /label=BBa_B0010
     terminator      2523..2602
                     /label=BBa_B0010
     stem_loop       2618..2637
                     /label=T7 TE
     misc_feature    2644..2644
                     /label=stop
     polyA_site      2638..2651
                     /label=polya
     misc_feature    2611..2651
                     /label=BBa_B0012
     terminator      2611..2651
                     /label=BBa_B0012
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt gcatgcgtta ttacagagta atcaggccca ggttaacggc cataatcgct
      181 gcaacgcggc gagaagtaac accgaattta cgacgaatgt tacccatgtg gaagttgacg
      241 ttggcttcag agcagttgca aattacagaa atctcccagg aggttttgcc gatagcgcac
      301 cattgcagga cctctttttc acggctggtc agaactaccg gtttagaaac tgggtgttcg
      361 aacgccagac ccgcgccgga ttgcagtgca taatctttca gcatccacag ggtcggcagt
      421 acagattcga tgaaacggtt agcctccgcg cggttttctg cttctacgga cagagacaga
      481 gcgcccagtt cgccacgagc accatgcagc ggcatcgtca ggccgtaaac cagaccagcc
      541 gcggacgcct cttcgaagaa ctcgtgctgt ttacgggtct ggtagatgct cggttcccag
      601 aaaatcggca gcacggactg ggtgcaatgg gaaacggttg gatctacacg tgcataaccg
      661 gcacgatcat aatgttcacg ccacgcggcc gggtaattgc ctacgatgaa tgcgttttcg
      721 tagtcttggg aatctttcgg cagcaggccg aacaggattt tagagaaacc caggtcagag
      781 gccatttttt gcaggatagc gctccattcc agtttaccag aagaacgttc cagttccaga
      841 aaaccatcca ccagagccat ctagtatttc tcctcttttg gatccggcaa ccattatcac
      901 cgccagaggt aaaatagtca acacgcacgg tgttattcat gaggcccctc gctgagcgcg
      961 tcccggagct gggggcaacc tagctgccac ctgcttttct gctagctatt ccagcgaaaa
     1021 catacagatt tccggcgaaa tcaaggctac ctgccagttc tggcaggttt ggccgcgggt
     1081 tctttttggt acacgaaagc tgtcgacgaa agaggagaaa tactagatga ttgttcagat
     1141 tggccgtcgt gaggaatttg ataaaaagct gctgggcgag atgcacaaac tgcgtgcaca
     1201 ggttttcaag gagcgtaaag gttgggacgt tagcgttatt gacgaaatgg agattgatgg
     1261 ctacgacgct ctgtctccgt attatatgct gattcaggag gacaccccgg aagctcaggt
     1321 atttggttgc tggcgtatct tcgatactac gggtccgtat atgctgaaaa ataccttccc
     1381 ggaactgctg cacggtaaag aagcgccttg tagcccgcac atctgggaac tgtctcgttt
     1441 cgctatcaac tccggccaga aaggttccct gggcttttcc gattgtaccc tggaagcaat
     1501 gcgtgcgctg gcgcgctatt ccctgcaaaa cgacatccag actctggtga ccgttactac
     1561 tgtgggcgtt gaaaagatga tgatccgtgc aggcctggac gttagccgtt tcggtccaca
     1621 cctgaagatc ggtatcgaac gcgcggtggc cctgcgtatc gaactgaatg caaaaaccca
     1681 gatcgcactg tatggtggtg ttctggtgga acaacgcctg gccgtttccg cggctaatga
     1741 tgagaactac gcgctggtag cataataata ctagagaaag aggagaaata ctagatgcgt
     1801 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1861 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1921 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1981 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     2041 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     2101 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     2161 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     2221 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     2281 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     2341 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     2401 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     2461 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     2521 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2581 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2641 ctgcgtttat a
//