##gff-version 3
##sequence-region BBa_J69101 1 2651
BBa_J69101	.	ribosome_entry_site	1777	1788	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J69101	.	conserved_region	1781	1784	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69101	.	engineered_region	1	1768	.	+	0	ID=BBa_J28032;Name=BBa_J28032
BBa_J69101	.	CDS	1127	1768	.	+	0	ID=annotation1904959;Name=BBa_C0078 (lasI), codons optimised for E. coli;Parent=BBa_J28032
BBa_J69101	.	stem_loop	1	129	.	+	0	ID=annotation1904949;Name=BBa_B0015;Parent=BBa_J28032
BBa_J69101	.	CDS	138	860	.	+	0	ID=annotation1904951;Name=BBa_C0079 (lasR), codons optimised for E. coli;Parent=BBa_J28032
BBa_J69101	.	ribosome_entry_site	1109	1120	.	+	0	ID=annotation1904958;Name=BBa_B0034;Parent=BBa_J28032
BBa_J69101	.	sequence_feature	131	136	.	+	0	ID=annotation1904950;Name=SphI restriction site;Parent=BBa_J28032
BBa_J69101	.	promoter	944	1100	.	+	0	ID=annotation1904956;Name=BBa_R0079 (las pR);Parent=BBa_J28032
BBa_J69101	.	sequence_feature	880	885	.	+	0	ID=annotation1904953;Name=BamHI restriction site;Parent=BBa_J28032
BBa_J69101	.	ribosome_entry_site	867	878	.	+	0	ID=annotation1904952;Name=BBa_B0034;Parent=BBa_J28032
BBa_J69101	.	engineered_region	1	1768	.	+	0	ID=annotation1904960;Name=BBa_J28032;Parent=BBa_J28032
BBa_J69101	.	promoter	887	935	.	+	0	ID=annotation1904954;Name=BBa_R0051 (lamda cI);Parent=BBa_J28032
BBa_J69101	.	sequence_feature	937	942	.	+	0	ID=annotation1904955;Name=BspHI restriction site;Parent=BBa_J28032
BBa_J69101	.	sequence_feature	1102	1107	.	+	0	ID=annotation1904957;Name=SalI restriction site;Parent=BBa_J28032
BBa_J69101	.	CDS	1795	2514	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J69101	.	CDS	1795	2514	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J69101	.	terminator	2611	2651	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J69101	.	engineered_region	2611	2651	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69101	.	polyA_site	2638	2651	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69101	.	stop_codon	2644	2644	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69101	.	stem_loop	2618	2637	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69101	.	terminator	2523	2602	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J69101	.	engineered_region	2523	2602	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69101	.	stem_loop	2534	2577	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J69101
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcc
caggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttg
acgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctt
tttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatc
tttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacaga
gacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctctt
cgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatggga
aacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaat
gcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccatttttt
gcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtattt
ctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccc
tcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacag
atttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcga
cgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgca
caaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctac
gacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcg
atactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacat
ctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatg
cgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatga
tgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtat
cgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaat
gatgagaactacgcgctggtagcataataatactagagaaagaggagaaatactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata