##gff-version 3
##sequence-region BBa_J69103 1 2518
BBa_J69103	.	engineered_region	1	1768	.	+	0	ID=BBa_J28032;Name=BBa_J28032
BBa_J69103	.	engineered_region	1	1768	.	+	0	ID=annotation1904960;Name=BBa_J28032;Parent=BBa_J28032
BBa_J69103	.	CDS	1127	1768	.	+	0	ID=annotation1904959;Name=BBa_C0078 (lasI), codons optimised for E. coli;Parent=BBa_J28032
BBa_J69103	.	sequence_feature	131	136	.	+	0	ID=annotation1904950;Name=SphI restriction site;Parent=BBa_J28032
BBa_J69103	.	ribosome_entry_site	867	878	.	+	0	ID=annotation1904952;Name=BBa_B0034;Parent=BBa_J28032
BBa_J69103	.	sequence_feature	937	942	.	+	0	ID=annotation1904955;Name=BspHI restriction site;Parent=BBa_J28032
BBa_J69103	.	ribosome_entry_site	1109	1120	.	+	0	ID=annotation1904958;Name=BBa_B0034;Parent=BBa_J28032
BBa_J69103	.	promoter	944	1100	.	+	0	ID=annotation1904956;Name=BBa_R0079 (las pR);Parent=BBa_J28032
BBa_J69103	.	stem_loop	1	129	.	+	0	ID=annotation1904949;Name=BBa_B0015;Parent=BBa_J28032
BBa_J69103	.	CDS	138	860	.	+	0	ID=annotation1904951;Name=BBa_C0079 (lasR), codons optimised for E. coli;Parent=BBa_J28032
BBa_J69103	.	sequence_feature	880	885	.	+	0	ID=annotation1904953;Name=BamHI restriction site;Parent=BBa_J28032
BBa_J69103	.	promoter	887	935	.	+	0	ID=annotation1904954;Name=BBa_R0051 (lamda cI);Parent=BBa_J28032
BBa_J69103	.	sequence_feature	1102	1107	.	+	0	ID=annotation1904957;Name=SalI restriction site;Parent=BBa_J28032
BBa_J69103	.	ribosome_entry_site	1777	1789	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J69103	.	ribosome_entry_site	1783	1786	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J69103	.	ribosome_entry_site	1777	1789	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J69103	.	engineered_region	1777	1789	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J69103	.	CDS	1796	2518	.	+	0	ID=BBa_E0030;Name=BBa_E0030
>BBa_J69103
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcc
caggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttg
acgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctt
tttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatc
tttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacaga
gacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctctt
cgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatggga
aacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaat
gcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccatttttt
gcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtattt
ctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccc
tcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacag
atttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcga
cgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgca
caaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctac
gacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcg
atactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacat
ctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatg
cgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatga
tgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtat
cgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaat
gatgagaactacgcgctggtagcataataatactagagtcacacaggaaagtactagatggtgagcaagggcgaggagc
tgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgaggg
cgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccacc
ctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagt
ccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggt
gaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctgggg
cacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaact
tcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacgg
ccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcac
atggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa