BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation2214014 1 Help:Barcodes range2214014 1 682 706 annotation1014044 1 mrfp1 range1014044 1 1 675 BBa_J69104 1 BBa_J69104 LasAIC with RFP output 2007-02-22T12:00:00Z 2015-05-08T01:08:18Z LasAIC with RFP output LasAIC with RFP output false false _101_ 0 740 101 Not in stock false NA false James Brown component2245416 1 BBa_B0034 component2245403 1 BBa_J28032 component2245419 1 BBa_E1010 annotation2245419 1 BBa_E1010 range2245419 1 1795 2500 annotation2245416 1 BBa_B0034 range2245416 1 1777 1788 annotation2245403 1 BBa_J28032 range2245403 1 1 1768 BBa_J28032 1 BBa_J28032 las autoinducer 2006-10-24T11:00:00Z 2015-08-31T04:08:44Z soon soon false false _101_ 0 1072 57 It's complicated true soon true Cambridge iGEM 2006 Team annotation1904951 1 BBa_C0079 (lasR), codons optimised for E. coli range1904951 1 138 860 annotation1904957 1 SalI restriction site range1904957 1 1102 1107 annotation1904956 1 BBa_R0079 (las pR) range1904956 1 944 1100 annotation1904954 1 BBa_R0051 (lamda cI) range1904954 1 887 935 annotation1904958 1 BBa_B0034 range1904958 1 1109 1120 annotation1904950 1 SphI restriction site range1904950 1 131 136 annotation1904955 1 BspHI restriction site range1904955 1 937 942 annotation1904952 1 BBa_B0034 range1904952 1 867 878 annotation1904953 1 BamHI restriction site range1904953 1 880 885 annotation1904960 1 BBa_J28032 range1904960 1 1 1768 annotation1904949 1 BBa_B0015 range1904949 1 1 129 annotation1904959 1 BBa_C0078 (lasI), codons optimised for E. coli range1904959 1 1127 1768 BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_J69104_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcgacgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgcacaaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctacgacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcgatactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacatctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatgcgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatgatgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtatcgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaatgatgagaactacgcgctggtagcataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_J28032_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcgacgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgcacaaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctacgacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcgatactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacatctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatgcgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatgatgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtatcgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaatgatgagaactacgcgctggtagcataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z