LOCUS BBa_J69105 2514 bp DNA linear 20-Dec-2024 DEFINITION LasAIC with GFP output ACCESSION BBa_J69105 VERSION BBa_J69105.1 FEATURES Location/Qualifiers stem_loop 1..129 /label=BBa_B0015 misc_feature 131..136 /label=SphI restriction site CDS 138..860 /label=BBa_C0079 (lasR), codons optimised for E. coli RBS 867..878 /label=BBa_B0034 misc_feature 880..885 /label=BamHI restriction site promoter 887..935 /label=BBa_R0051 (lamda cI) misc_feature 937..942 /label=BspHI restriction site promoter 944..1100 /label=BBa_R0079 (las pR) misc_feature 1102..1107 /label=SalI restriction site RBS 1109..1120 /label=BBa_B0034 CDS 1127..1768 /label=BBa_C0078 (lasI), codons optimised for E. coli misc_feature 1..1768 /label=BBa_J28032 misc_feature 1..1768 /label=BBa_J28032 misc_feature 1781..1784 /label=conserved RBS 1777..1788 /label=BBa_B0034 CDS 1795..2514 /label=GFP protein CDS 1795..2514 /label=BBa_E0040 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt gcatgcgtta ttacagagta atcaggccca ggttaacggc cataatcgct 181 gcaacgcggc gagaagtaac accgaattta cgacgaatgt tacccatgtg gaagttgacg 241 ttggcttcag agcagttgca aattacagaa atctcccagg aggttttgcc gatagcgcac 301 cattgcagga cctctttttc acggctggtc agaactaccg gtttagaaac tgggtgttcg 361 aacgccagac ccgcgccgga ttgcagtgca taatctttca gcatccacag ggtcggcagt 421 acagattcga tgaaacggtt agcctccgcg cggttttctg cttctacgga cagagacaga 481 gcgcccagtt cgccacgagc accatgcagc ggcatcgtca ggccgtaaac cagaccagcc 541 gcggacgcct cttcgaagaa ctcgtgctgt ttacgggtct ggtagatgct cggttcccag 601 aaaatcggca gcacggactg ggtgcaatgg gaaacggttg gatctacacg tgcataaccg 661 gcacgatcat aatgttcacg ccacgcggcc gggtaattgc ctacgatgaa tgcgttttcg 721 tagtcttggg aatctttcgg cagcaggccg aacaggattt tagagaaacc caggtcagag 781 gccatttttt gcaggatagc gctccattcc agtttaccag aagaacgttc cagttccaga 841 aaaccatcca ccagagccat ctagtatttc tcctcttttg gatccggcaa ccattatcac 901 cgccagaggt aaaatagtca acacgcacgg tgttattcat gaggcccctc gctgagcgcg 961 tcccggagct gggggcaacc tagctgccac ctgcttttct gctagctatt ccagcgaaaa 1021 catacagatt tccggcgaaa tcaaggctac ctgccagttc tggcaggttt ggccgcgggt 1081 tctttttggt acacgaaagc tgtcgacgaa agaggagaaa tactagatga ttgttcagat 1141 tggccgtcgt gaggaatttg ataaaaagct gctgggcgag atgcacaaac tgcgtgcaca 1201 ggttttcaag gagcgtaaag gttgggacgt tagcgttatt gacgaaatgg agattgatgg 1261 ctacgacgct ctgtctccgt attatatgct gattcaggag gacaccccgg aagctcaggt 1321 atttggttgc tggcgtatct tcgatactac gggtccgtat atgctgaaaa ataccttccc 1381 ggaactgctg cacggtaaag aagcgccttg tagcccgcac atctgggaac tgtctcgttt 1441 cgctatcaac tccggccaga aaggttccct gggcttttcc gattgtaccc tggaagcaat 1501 gcgtgcgctg gcgcgctatt ccctgcaaaa cgacatccag actctggtga ccgttactac 1561 tgtgggcgtt gaaaagatga tgatccgtgc aggcctggac gttagccgtt tcggtccaca 1621 cctgaagatc ggtatcgaac gcgcggtggc cctgcgtatc gaactgaatg caaaaaccca 1681 gatcgcactg tatggtggtg ttctggtgga acaacgcctg gccgtttccg cggctaatga 1741 tgagaactac gcgctggtag cataataata ctagagaaag aggagaaata ctagatgcgt 1801 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 1861 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 1921 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 1981 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 2041 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 2101 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 2161 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 2221 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa 2281 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat 2341 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc 2401 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag 2461 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataa //