BBa_J69105 1 BBa_J69105 LasAIC with GFP output 2007-02-22T12:00:00Z 2015-05-08T01:08:18Z LasAIC with GFP output LasAIC with GFP output false false _101_ 0 740 101 Not in stock false NA false James Brown component1912180 1 BBa_B0034 component1912181 1 BBa_E0040 component1912167 1 BBa_J28032 annotation1912181 1 BBa_E0040 range1912181 1 1795 2514 annotation1912167 1 BBa_J28032 range1912167 1 1 1768 annotation1912180 1 BBa_B0034 range1912180 1 1777 1788 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J28032 1 BBa_J28032 las autoinducer 2006-10-24T11:00:00Z 2015-08-31T04:08:44Z soon soon false false _101_ 0 1072 57 It's complicated true soon true Cambridge iGEM 2006 Team annotation1904951 1 BBa_C0079 (lasR), codons optimised for E. coli range1904951 1 138 860 annotation1904949 1 BBa_B0015 range1904949 1 1 129 annotation1904957 1 SalI restriction site range1904957 1 1102 1107 annotation1904950 1 SphI restriction site range1904950 1 131 136 annotation1904958 1 BBa_B0034 range1904958 1 1109 1120 annotation1904955 1 BspHI restriction site range1904955 1 937 942 annotation1904954 1 BBa_R0051 (lamda cI) range1904954 1 887 935 annotation1904953 1 BamHI restriction site range1904953 1 880 885 annotation1904956 1 BBa_R0079 (las pR) range1904956 1 944 1100 annotation1904952 1 BBa_B0034 range1904952 1 867 878 annotation1904960 1 BBa_J28032 range1904960 1 1 1768 annotation1904959 1 BBa_C0078 (lasI), codons optimised for E. coli range1904959 1 1127 1768 BBa_J69105_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcgacgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgcacaaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctacgacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcgatactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacatctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatgcgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatgatgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtatcgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaatgatgagaactacgcgctggtagcataataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J28032_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcgacgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgcacaaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctacgacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcgatactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacatctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatgcgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatgatgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtatcgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaatgatgagaactacgcgctggtagcataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z