BBa_J06504 1 mCherry monomeric RFP optimized for bacteria 2005-07-17T11:00:00Z 2016-01-25T01:05:28Z mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible Released HQ 2013 mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends. false false _20_ 4206 340 20 In stock false <p> Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. </p> <p> Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick. </p> true ytwang annotation1585833 1 C->T (removing PstI site) range1585833 1 352 352 annotation1585829 1 mCherry range1585829 1 1 711 BBa_J28032 1 BBa_J28032 las autoinducer 2006-10-24T11:00:00Z 2015-08-31T04:08:44Z soon soon false false _101_ 0 1072 57 It's complicated true soon true Cambridge iGEM 2006 Team annotation1904959 1 BBa_C0078 (lasI), codons optimised for E. coli range1904959 1 1127 1768 annotation1904957 1 SalI restriction site range1904957 1 1102 1107 annotation1904951 1 BBa_C0079 (lasR), codons optimised for E. coli range1904951 1 138 860 annotation1904952 1 BBa_B0034 range1904952 1 867 878 annotation1904954 1 BBa_R0051 (lamda cI) range1904954 1 887 935 annotation1904958 1 BBa_B0034 range1904958 1 1109 1120 annotation1904956 1 BBa_R0079 (las pR) range1904956 1 944 1100 annotation1904949 1 BBa_B0015 range1904949 1 1 129 annotation1904950 1 SphI restriction site range1904950 1 131 136 annotation1904955 1 BspHI restriction site range1904955 1 937 942 annotation1904953 1 BamHI restriction site range1904953 1 880 885 annotation1904960 1 BBa_J28032 range1904960 1 1 1768 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J69106 1 BBa_J69106 LasAIC with mCherry output 2007-02-22T12:00:00Z 2015-05-08T01:08:18Z LasAIC with mCherry output LasAIC with mCherry output false false _101_ 0 740 101 Not in stock false NA false James Brown component1912195 1 BBa_B0034 component1912198 1 BBa_J06504 component1912182 1 BBa_J28032 annotation1912195 1 BBa_B0034 range1912195 1 1777 1788 annotation1912182 1 BBa_J28032 range1912182 1 1 1768 annotation1912198 1 BBa_J06504 range1912198 1 1795 2508 BBa_B0034_sequence 1 aaagaggagaaa BBa_J06504_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_J69106_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcgacgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgcacaaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctacgacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcgatactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacatctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatgcgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatgatgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtatcgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaatgatgagaactacgcgctggtagcataataatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_J28032_sequence 1 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcccaggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttgacgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctttttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatctttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacagagacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctcttcgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatgggaaacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaatgcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccattttttgcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtatttctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcgacgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgcacaaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctacgacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcgatactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacatctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatgcgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatgatgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtatcgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaatgatgagaactacgcgctggtagcataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z