LOCUS       BBa_J69107              2645 bp    DNA     linear       02-May-2024
DEFINITION  LasAIC with Terminated mCherry output
ACCESSION   BBa_J69107
VERSION     BBa_J69107.1  
FEATURES             Location/Qualifiers
     stem_loop       1..129
                     /label=BBa_B0015
     misc_feature    131..136
                     /label=SphI restriction site
     CDS             138..860
                     /label=BBa_C0079 (lasR), codons optimised for E. coli
     RBS             867..878
                     /label=BBa_B0034
     misc_feature    880..885
                     /label=BamHI restriction site
     promoter        887..935
                     /label=BBa_R0051 (lamda cI)
     misc_feature    937..942
                     /label=BspHI restriction site
     promoter        944..1100
                     /label=BBa_R0079 (las pR)
     misc_feature    1102..1107
                     /label=SalI restriction site
     RBS             1109..1120
                     /label=BBa_B0034
     CDS             1127..1768
                     /label=BBa_C0078 (lasI), codons optimised for E. coli
     misc_feature    1..1768
                     /label=BBa_J28032
     misc_feature    1..1768
                     /label=BBa_J28032
     misc_feature    1781..1784
                     /label=conserved
     RBS             1777..1788
                     /label=BBa_B0034
     CDS             1795..2505
                     /label=mCherry
     misc_feature    2146..2146
                     /label=C->T (removing PstI site)
     misc_feature    1795..2508
                     /label=BBa_J06504
     stem_loop       2528..2571
                     /label=stem_loop
     misc_feature    2517..2596
                     /label=BBa_B0010
     terminator      2517..2596
                     /label=BBa_B0010
     stem_loop       2612..2631
                     /label=T7 TE
     misc_feature    2638..2638
                     /label=stop
     polyA_site      2632..2645
                     /label=polya
     misc_feature    2605..2645
                     /label=BBa_B0012
     terminator      2605..2645
                     /label=BBa_B0012
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt gcatgcgtta ttacagagta atcaggccca ggttaacggc cataatcgct
      181 gcaacgcggc gagaagtaac accgaattta cgacgaatgt tacccatgtg gaagttgacg
      241 ttggcttcag agcagttgca aattacagaa atctcccagg aggttttgcc gatagcgcac
      301 cattgcagga cctctttttc acggctggtc agaactaccg gtttagaaac tgggtgttcg
      361 aacgccagac ccgcgccgga ttgcagtgca taatctttca gcatccacag ggtcggcagt
      421 acagattcga tgaaacggtt agcctccgcg cggttttctg cttctacgga cagagacaga
      481 gcgcccagtt cgccacgagc accatgcagc ggcatcgtca ggccgtaaac cagaccagcc
      541 gcggacgcct cttcgaagaa ctcgtgctgt ttacgggtct ggtagatgct cggttcccag
      601 aaaatcggca gcacggactg ggtgcaatgg gaaacggttg gatctacacg tgcataaccg
      661 gcacgatcat aatgttcacg ccacgcggcc gggtaattgc ctacgatgaa tgcgttttcg
      721 tagtcttggg aatctttcgg cagcaggccg aacaggattt tagagaaacc caggtcagag
      781 gccatttttt gcaggatagc gctccattcc agtttaccag aagaacgttc cagttccaga
      841 aaaccatcca ccagagccat ctagtatttc tcctcttttg gatccggcaa ccattatcac
      901 cgccagaggt aaaatagtca acacgcacgg tgttattcat gaggcccctc gctgagcgcg
      961 tcccggagct gggggcaacc tagctgccac ctgcttttct gctagctatt ccagcgaaaa
     1021 catacagatt tccggcgaaa tcaaggctac ctgccagttc tggcaggttt ggccgcgggt
     1081 tctttttggt acacgaaagc tgtcgacgaa agaggagaaa tactagatga ttgttcagat
     1141 tggccgtcgt gaggaatttg ataaaaagct gctgggcgag atgcacaaac tgcgtgcaca
     1201 ggttttcaag gagcgtaaag gttgggacgt tagcgttatt gacgaaatgg agattgatgg
     1261 ctacgacgct ctgtctccgt attatatgct gattcaggag gacaccccgg aagctcaggt
     1321 atttggttgc tggcgtatct tcgatactac gggtccgtat atgctgaaaa ataccttccc
     1381 ggaactgctg cacggtaaag aagcgccttg tagcccgcac atctgggaac tgtctcgttt
     1441 cgctatcaac tccggccaga aaggttccct gggcttttcc gattgtaccc tggaagcaat
     1501 gcgtgcgctg gcgcgctatt ccctgcaaaa cgacatccag actctggtga ccgttactac
     1561 tgtgggcgtt gaaaagatga tgatccgtgc aggcctggac gttagccgtt tcggtccaca
     1621 cctgaagatc ggtatcgaac gcgcggtggc cctgcgtatc gaactgaatg caaaaaccca
     1681 gatcgcactg tatggtggtg ttctggtgga acaacgcctg gccgtttccg cggctaatga
     1741 tgagaactac gcgctggtag cataataata ctagagaaag aggagaaata ctagatggtg
     1801 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     1861 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     1921 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     1981 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     2041 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     2101 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag
     2161 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     2221 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     2281 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     2341 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     2401 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     2461 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaataata ctagagccag
     2521 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2581 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2641 ttata
//