LOCUS       BBa_J692101             1123 bp    DNA     linear       19-Dec-2024
DEFINITION  CviR-vioA Receiver v2.00 Characterisation Device (GFP)
ACCESSION   BBa_J692101
VERSION     BBa_J692101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..86
                     /label=BBa_C0099
     stem_loop       106..149
                     /label=stem_loop
     misc_feature    95..174
                     /label=BBa_B0010
     terminator      95..174
                     /label=BBa_B0010
     stem_loop       190..209
                     /label=T7 TE
     misc_feature    216..216
                     /label=stop
     polyA_site      210..223
                     /label=polya
     misc_feature    183..223
                     /label=BBa_B0012
     terminator      183..223
                     /label=BBa_B0012
     promoter        232..240
                     /label=BBa_R0099
     misc_feature    253..256
                     /label=conserved
     RBS             249..260
                     /label=BBa_B0034
     CDS             267..986
                     /label=GFP protein
     CDS             267..986
                     /label=BBa_E0040
     stem_loop       1006..1049
                     /label=stem_loop
     misc_feature    995..1074
                     /label=BBa_B0010
     terminator      995..1074
                     /label=BBa_B0010
     stem_loop       1090..1109
                     /label=T7 TE
     misc_feature    1116..1116
                     /label=stop
     polyA_site      1110..1123
                     /label=polya
     misc_feature    1083..1123
                     /label=BBa_B0012
     terminator      1083..1123
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatgtact agagccaggc atcaaataaa acgaaaggct
      121 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      181 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gcgtcgtcgt
      241 tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
      301 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      361 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      421 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
      481 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
      541 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
      601 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
      661 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
      721 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
      781 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
      841 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
      901 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
      961 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     1021 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1081 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//