##gff-version 3
##sequence-region BBa_J69212 1 241
BBa_J69212	.	terminator	184	224	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J69212	.	engineered_region	184	224	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69212	.	stop_codon	217	217	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69212	.	stem_loop	191	210	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69212	.	polyA_site	211	224	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69212	.	promoter	233	241	.	+	0	ID=BBa_R0099;Name=BBa_R0099
BBa_J69212	.	terminator	96	175	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J69212	.	stem_loop	107	150	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69212	.	engineered_region	96	175	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69212	.	CDS	82	87	.	+	0	ID=BBa_C0099;Name=BBa_C0099
BBa_J69212	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J69212	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J69212	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J69212	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J69212	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J69212	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J69212	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J69212	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J69212	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J69212	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_J69212
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgtcg
tcgt