##gff-version 3
##sequence-region BBa_J69512 1 932
BBa_J69512	.	engineered_region	82	795	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J69512	.	sequence_alteration	433	433	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J69512	.	CDS	82	792	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J69512	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J69512	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69512	.	terminator	804	883	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J69512	.	stem_loop	815	858	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69512	.	engineered_region	804	883	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69512	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J69512	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J69512	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J69512	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J69512	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J69512	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J69512	.	terminator	892	932	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J69512	.	engineered_region	892	932	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69512	.	stop_codon	925	925	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69512	.	stem_loop	899	918	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69512	.	polyA_site	919	932	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J69512
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata