LOCUS       BBa_J69516               903 bp    DNA     linear       22-Nov-2024
DEFINITION  lacI-regulated tetR invertor Protein Generator
ACCESSION   BBa_J69516
VERSION     BBa_J69516.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..701
                     /label=tetR
     misc_feature    702..735
                     /label=SsrA
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             82..741
                     /label=BBa_C0040
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     misc_feature    64..903
                     /label=BBa_P0440
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 ata
//