LOCUS       BBa_J69522               931 bp    DNA     linear       05-Nov-2024
DEFINITION  tetR-repressible promoter coupled with mCherry Reporter
ACCESSION   BBa_J69522
VERSION     BBa_J69522.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..791
                     /label=mCherry
     misc_feature    432..432
                     /label=C->T (removing PstI site)
     misc_feature    81..794
                     /label=BBa_J06504
     stem_loop       814..857
                     /label=stem_loop
     misc_feature    803..882
                     /label=BBa_B0010
     terminator      803..882
                     /label=BBa_B0010
     stem_loop       898..917
                     /label=T7 TE
     misc_feature    924..924
                     /label=stop
     polyA_site      918..931
                     /label=polya
     misc_feature    891..931
                     /label=BBa_B0012
     terminator      891..931
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga ggataacatg gccatcatca
      121 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
      181 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      241 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
      301 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
      361 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
      421 aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
      481 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
      541 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
      601 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
      661 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
      721 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
      781 tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      841 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      901 tcaccttcgg gtgggccttt ctgcgtttat a
//