LOCUS BBa_J69536 2058 bp DNA linear 25-Nov-2024 DEFINITION Arabinose-inducible tetR Generator ACCESSION BBa_J69536 VERSION BBa_J69536.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1240..1856 /label=tetR misc_feature 1857..1890 /label=SsrA misc_feature 1897..1921 /label=Help:Barcodes CDS 1237..1896 /label=BBa_C0040 stem_loop 1941..1984 /label=stem_loop misc_feature 1930..2009 /label=BBa_B0010 terminator 1930..2009 /label=BBa_B0010 stem_loop 2025..2044 /label=T7 TE misc_feature 2051..2051 /label=stop polyA_site 2045..2058 /label=polya misc_feature 2018..2058 /label=BBa_B0012 terminator 2018..2058 /label=BBa_B0012 misc_feature 1219..2058 /label=BBa_P0440 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 1261 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 1321 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 1381 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 1441 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 1501 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 1561 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 1621 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 1681 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 1741 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 1801 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 1861 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 1921 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 1981 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2041 ggcctttctg cgtttata //