LOCUS       BBa_J69551              1040 bp    DNA     linear       13-Jun-2024
DEFINITION  plasB promoter - GFP
ACCESSION   BBa_J69551
VERSION     BBa_J69551.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     misc_feature    170..173
                     /label=conserved
     RBS             166..177
                     /label=BBa_B0034
     CDS             184..903
                     /label=GFP protein
     CDS             184..903
                     /label=BBa_E0040
     stem_loop       923..966
                     /label=stem_loop
     misc_feature    912..991
                     /label=BBa_B0010
     terminator      912..991
                     /label=BBa_B0010
     stem_loop       1007..1026
                     /label=T7 TE
     misc_feature    1033..1033
                     /label=stop
     polyA_site      1027..1040
                     /label=polya
     misc_feature    1000..1040
                     /label=BBa_B0012
     terminator      1000..1040
                     /label=BBa_B0012
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagaaaga ggagaaatac
      181 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      241 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      301 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      361 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      421 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      481 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      541 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      601 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      661 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      721 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      781 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      841 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      901 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      961 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1021 tgggcctttc tgcgtttata
//