LOCUS       BBa_J69604              1881 bp    DNA     linear       29-Jun-2024
DEFINITION  lacI Inverter Test Construct A
ACCESSION   BBa_J69604
VERSION     BBa_J69604.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..798
                     /label=BBa_E0030
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
     terminator      807..935
                     /label=BBa_B0015
     misc_feature    1..935
                     /label=BBa_J69504
     misc_feature    946..962
                     /label=lac O1
     promoter        963..968
                     /label=-35
     misc_feature    969..985
                     /label=lac O1
     promoter        986..991
                     /label=-10
     misc_feature    944..997
                     /label=BBa_R0011
     promoter        944..997
                     /label=BBa_R0011
     misc_feature    1011..1014
                     /label=conserved
     RBS             1007..1018
                     /label=BBa_B0034
     CDS             1025..1744
                     /label=GFP protein
     CDS             1025..1744
                     /label=BBa_E0040
     stem_loop       1764..1807
                     /label=stem_loop
     misc_feature    1753..1832
                     /label=BBa_B0010
     terminator      1753..1832
                     /label=BBa_B0010
     stem_loop       1848..1867
                     /label=T7 TE
     misc_feature    1874..1874
                     /label=stop
     polyA_site      1868..1881
                     /label=polya
     misc_feature    1841..1881
                     /label=BBa_B0012
     terminator      1841..1881
                     /label=BBa_B0012
     misc_feature    944..1881
                     /label=BBa_J69511
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac
      961 aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata
     1021 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1081 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1141 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1201 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1261 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1321 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1381 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1441 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1501 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1561 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1621 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1681 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     1741 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1801 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1861 gtgggccttt ctgcgtttat a
//