LOCUS       BBa_J69616              1848 bp    DNA     linear       25-Jun-2024
DEFINITION  tetR Inverter Test Construct B
ACCESSION   BBa_J69616
VERSION     BBa_J69616.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..701
                     /label=tetR
     misc_feature    702..735
                     /label=SsrA
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             82..741
                     /label=BBa_C0040
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     misc_feature    64..903
                     /label=BBa_P0440
     misc_feature    1..903
                     /label=BBa_J69516
     misc_feature    912..930
                     /label=TetR 1
     misc_feature    912..965
                     /label=BBa_R0040
     promoter        931..936
                     /label=-35
     misc_feature    937..955
                     /label=TetR 2
     promoter        954..959
                     /label=-10
     promoter        912..965
                     /label=BBa_R0040
     misc_feature    978..981
                     /label=conserved
     RBS             974..985
                     /label=BBa_B0034
     CDS             992..1711
                     /label=GFP protein
     CDS             992..1711
                     /label=BBa_E0040
     stem_loop       1731..1774
                     /label=stem_loop
     misc_feature    1720..1799
                     /label=BBa_B0010
     terminator      1720..1799
                     /label=BBa_B0010
     stem_loop       1815..1834
                     /label=T7 TE
     misc_feature    1841..1841
                     /label=stop
     polyA_site      1835..1848
                     /label=polya
     misc_feature    1808..1848
                     /label=BBa_B0012
     terminator      1808..1848
                     /label=BBa_B0012
     misc_feature    912..1848
                     /label=BBa_J69521
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
      961 agcactacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
     1021 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1081 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1141 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     1201 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1261 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1321 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1381 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     1441 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     1501 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1561 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1621 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1681 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     1741 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1801 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//