LOCUS       BBa_J69666              1849 bp    DNA     linear       21-Jun-2024
DEFINITION  Potentially J69516 with J69511
ACCESSION   BBa_J69666
VERSION     BBa_J69666.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             85..701
                     /label=tetR
     misc_feature    702..735
                     /label=SsrA
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             82..741
                     /label=BBa_C0040
     stem_loop       786..829
                     /label=stem_loop
     misc_feature    775..854
                     /label=BBa_B0010
     terminator      775..854
                     /label=BBa_B0010
     stem_loop       870..889
                     /label=T7 TE
     misc_feature    896..896
                     /label=stop
     polyA_site      890..903
                     /label=polya
     misc_feature    863..903
                     /label=BBa_B0012
     terminator      863..903
                     /label=BBa_B0012
     misc_feature    64..903
                     /label=BBa_P0440
     misc_feature    1..903
                     /label=BBa_J69516
     misc_feature    914..930
                     /label=lac O1
     promoter        931..936
                     /label=-35
     misc_feature    937..953
                     /label=lac O1
     promoter        954..959
                     /label=-10
     misc_feature    912..965
                     /label=BBa_R0011
     promoter        912..965
                     /label=BBa_R0011
     misc_feature    979..982
                     /label=conserved
     RBS             975..986
                     /label=BBa_B0034
     CDS             993..1712
                     /label=GFP protein
     CDS             993..1712
                     /label=BBa_E0040
     stem_loop       1732..1775
                     /label=stem_loop
     misc_feature    1721..1800
                     /label=BBa_B0010
     terminator      1721..1800
                     /label=BBa_B0010
     stem_loop       1816..1835
                     /label=T7 TE
     misc_feature    1842..1842
                     /label=stop
     polyA_site      1836..1849
                     /label=polya
     misc_feature    1809..1849
                     /label=BBa_B0012
     terminator      1809..1849
                     /label=BBa_B0012
     misc_feature    912..1849
                     /label=BBa_J69511
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
      301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
      361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
      421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
      481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
      541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
      601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
      661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
      781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      901 atatactaga gaattgtgag cggataacaa ttgacattgt gagcggataa caagatactg
      961 agcacatact agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg
     1021 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1081 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1141 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     1201 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     1261 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1321 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1381 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     1441 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     1501 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     1561 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     1621 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     1681 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
     1741 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1801 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//