LOCUS       BBa_J69705              2316 bp    DNA     linear       21-May-2024
DEFINITION  Available Part Name
ACCESSION   BBa_J69705
VERSION     BBa_J69705.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    1170..1208
                     /label=LVA
     CDS             81..1208
                     /label=lacI-LVA
     misc_feature    81..1208
                     /label=BBa_C0012
     misc_feature    1209..1233
                     /label=Help:Barcodes
     CDS             81..1208
                     /label=BBa_C0012
     stem_loop       1253..1296
                     /label=stem_loop
     misc_feature    1242..1321
                     /label=BBa_B0010
     terminator      1242..1321
                     /label=BBa_B0010
     stem_loop       1337..1356
                     /label=T7 TE
     misc_feature    1363..1363
                     /label=stop
     polyA_site      1357..1370
                     /label=polya
     misc_feature    1330..1370
                     /label=BBa_B0012
     terminator      1330..1370
                     /label=BBa_B0012
     terminator      1242..1370
                     /label=BBa_B0015
     misc_feature    1381..1397
                     /label=lac O1
     promoter        1398..1403
                     /label=-35
     misc_feature    1404..1420
                     /label=lac O1
     promoter        1421..1426
                     /label=-10
     misc_feature    1379..1432
                     /label=BBa_R0011
     promoter        1379..1432
                     /label=BBa_R0011
     misc_feature    63..1433
                     /label=BBa_Q04121
     misc_feature    1446..1449
                     /label=conserved
     RBS             1442..1453
                     /label=BBa_B0034
     CDS             1460..2179
                     /label=GFP protein
     CDS             1460..2179
                     /label=BBa_E0040
     stem_loop       2199..2242
                     /label=stem_loop
     misc_feature    2188..2267
                     /label=BBa_B0010
     terminator      2188..2267
                     /label=BBa_B0010
     stem_loop       2283..2302
                     /label=T7 TE
     misc_feature    2309..2309
                     /label=stop
     polyA_site      2303..2316
                     /label=polya
     misc_feature    2276..2316
                     /label=BBa_B0012
     terminator      2276..2316
                     /label=BBa_B0012
     misc_feature    1442..2316
                     /label=BBa_I13504
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
      121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
      181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
      241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
      301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
      361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
      421 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
      481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
      541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
      601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
      661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
      721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
      781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
      841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
      901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
      961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     1201 cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc aaataaaacg
     1261 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1321 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagaa
     1381 ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc acatactaga
     1441 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
     1501 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1561 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1621 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1681 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1741 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1801 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1861 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1921 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1981 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     2041 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     2101 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2161 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     2221 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     2281 ctggctcacc ttcgggtggg cctttctgcg tttata
//