LOCUS       BBa_J69711              2408 bp    DNA     linear       22-Nov-2024
DEFINITION  lacI Inverter Test Device
ACCESSION   BBa_J69711
VERSION     BBa_J69711.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     misc_feature    162..165
                     /label=conserved
     RBS             158..169
                     /label=BBa_B0034
     misc_feature    1265..1303
                     /label=LVA
     CDS             176..1303
                     /label=lacI-LVA
     misc_feature    176..1303
                     /label=BBa_C0012
     misc_feature    1304..1328
                     /label=Help:Barcodes
     CDS             176..1303
                     /label=BBa_C0012
     stem_loop       1348..1391
                     /label=stem_loop
     misc_feature    1337..1416
                     /label=BBa_B0010
     terminator      1337..1416
                     /label=BBa_B0010
     stem_loop       1432..1451
                     /label=T7 TE
     misc_feature    1458..1458
                     /label=stop
     polyA_site      1452..1465
                     /label=polya
     misc_feature    1425..1465
                     /label=BBa_B0012
     terminator      1425..1465
                     /label=BBa_B0012
     misc_feature    158..1465
                     /label=BBa_P0412
     misc_feature    1..1465
                     /label=BBa_J69701
     promoter        1511..1516
                     /label=-10
     promoter        1488..1493
                     /label=-35
     misc_feature    1498..1514
                     /label=OR1
     misc_feature    1474..1490
                     /label=OR2
     misc_feature    1474..1522
                     /label=BBa_R0051
     promoter        1474..1522
                     /label=BBa_R0051
     misc_feature    1535..1538
                     /label=conserved
     RBS             1531..1542
                     /label=BBa_B0034
     CDS             1549..2271
                     /label=BBa_E0020
     stem_loop       2291..2334
                     /label=stem_loop
     misc_feature    2280..2359
                     /label=BBa_B0010
     terminator      2280..2359
                     /label=BBa_B0010
     stem_loop       2375..2394
                     /label=T7 TE
     misc_feature    2401..2401
                     /label=stop
     polyA_site      2395..2408
                     /label=polya
     misc_feature    2368..2408
                     /label=BBa_B0012
     terminator      2368..2408
                     /label=BBa_B0012
     terminator      2280..2408
                     /label=BBa_B0015
     misc_feature    1474..2408
                     /label=BBa_J69503
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagaaa gaggagaaat actagatggt
      181 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
      241 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
      301 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
      361 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
      421 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
      481 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
      541 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
      601 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
      661 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
      721 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
      781 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
      841 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
      901 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
      961 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     1021 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     1081 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     1141 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     1201 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1261 gcaggctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg
     1321 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1381 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1441 tcgggtgggc ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg actattttac
     1501 ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat ggtgagcaag
     1561 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
     1621 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1681 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1741 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1801 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1861 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1921 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1981 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     2041 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2101 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2161 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2221 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc
     2281 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2341 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2401 cgtttata
//