##gff-version 3 ##sequence-region BBa_J69711 1 2408 BBa_J69711 . engineered_region 1 1465 . + 0 ID=BBa_J69701;Name=BBa_J69701 BBa_J69711 . engineered_region 158 1465 . + 0 ID=BBa_P0412;Name=BBa_P0412;Parent=BBa_J69701 BBa_J69711 . CDS 176 1303 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412 BBa_J69711 . engineered_region 176 1303 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_J69711 . tag 1265 1303 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_J69711 . CDS 176 1303 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_J69711 . engineered_tag 1304 1328 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_J69711 . ribosome_entry_site 158 169 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412 BBa_J69711 . conserved_region 162 165 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J69711 . terminator 1425 1465 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412 BBa_J69711 . polyA_site 1452 1465 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J69711 . stop_codon 1458 1458 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J69711 . stem_loop 1432 1451 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J69711 . engineered_region 1425 1465 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J69711 . terminator 1337 1416 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412 BBa_J69711 . stem_loop 1348 1391 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J69711 . engineered_region 1337 1416 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J69711 . promoter 1 149 . + 0 ID=BBa_R0080;Name=BBa_R0080;Parent=BBa_J69701 BBa_J69711 . promoter 113 118 . + 0 ID=annotation308601;Name=-35;Parent=BBa_R0080 BBa_J69711 . promoter 136 141 . + 0 ID=annotation308602;Name=-10;Parent=BBa_R0080 BBa_J69711 . non_covalent_binding_site 4 29 . + 0 ID=annotation301456;Name=c-amp2;Parent=BBa_R0080 BBa_J69711 . non_covalent_binding_site 43 72 . + 0 ID=annotation301458;Name=c-amp1;Parent=BBa_R0080 BBa_J69711 . operator 6 44 . + 0 ID=annotation301457;Name=araO1;Parent=BBa_R0080 BBa_J69711 . non_covalent_binding_site 73 101 . + 0 ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080 BBa_J69711 . engineered_region 1474 2408 . + 0 ID=BBa_J69503;Name=BBa_J69503 BBa_J69711 . CDS 1549 2271 . + 0 ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_J69503 BBa_J69711 . promoter 1474 1522 . + 0 ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_J69503 BBa_J69711 . engineered_region 1474 1522 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_J69711 . promoter 1488 1493 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_J69711 . non_covalent_binding_site 1498 1514 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_J69711 . non_covalent_binding_site 1474 1490 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_J69711 . promoter 1511 1516 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_J69711 . ribosome_entry_site 1531 1542 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J69503 BBa_J69711 . conserved_region 1535 1538 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J69711 . terminator 2280 2408 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J69503 BBa_J69711 . terminator 2368 2408 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_J69711 . polyA_site 2395 2408 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J69711 . stop_codon 2401 2401 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J69711 . stem_loop 2375 2394 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J69711 . engineered_region 2368 2408 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J69711 . terminator 2280 2359 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_J69711 . stem_loop 2291 2334 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J69711 . engineered_region 2280 2359 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 >BBa_J69711 gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccata gcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagaga aagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatca gaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggag ctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtc tggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtc gatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatc attaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatg tctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgc attgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcat aaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaa ccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcg cgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgt tatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactct ctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatac gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggca tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacc tctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccgg ggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgat gccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacca ccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcc cgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgag ggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgg agtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccg ccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctg ctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgc tggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaa taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca cactggctcaccttcgggtgggcctttctgcgtttata