LOCUS       BBa_J69712              1858 bp    DNA     linear       22-Dec-2024
DEFINITION  lacI Inverter2 Test Device
ACCESSION   BBa_J69712
VERSION     BBa_J69712.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..792
                     /label=mCherry
     misc_feature    433..433
                     /label=C->T (removing PstI site)
     misc_feature    82..795
                     /label=BBa_J06504
     stem_loop       815..858
                     /label=stem_loop
     misc_feature    804..883
                     /label=BBa_B0010
     terminator      804..883
                     /label=BBa_B0010
     stem_loop       899..918
                     /label=T7 TE
     misc_feature    925..925
                     /label=stop
     polyA_site      919..932
                     /label=polya
     misc_feature    892..932
                     /label=BBa_B0012
     terminator      892..932
                     /label=BBa_B0012
     promoter        941..975
                     /label=BBa_J23101
     misc_feature    988..991
                     /label=conserved
     RBS             984..995
                     /label=BBa_B0034
     CDS             1002..1721
                     /label=GFP protein
     CDS             1002..1721
                     /label=BBa_E0040
     stem_loop       1741..1784
                     /label=stem_loop
     misc_feature    1730..1809
                     /label=BBa_B0010
     terminator      1730..1809
                     /label=BBa_B0010
     stem_loop       1825..1844
                     /label=T7 TE
     misc_feature    1851..1851
                     /label=stop
     polyA_site      1845..1858
                     /label=polya
     misc_feature    1818..1858
                     /label=BBa_B0012
     terminator      1818..1858
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
      121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      781 ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      901 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tttacagcta gctcagtcct
      961 aggtattatg ctagctacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac
     1021 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
     1081 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
     1141 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
     1201 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
     1261 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
     1321 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
     1381 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
     1441 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
     1501 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1561 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1621 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1681 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     1741 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1801 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//