LOCUS BBa_J69801 4864 bp DNA linear 22-Dec-2024 DEFINITION Lux Receiver with inducible aiiA Device ACCESSION BBa_J69801 VERSION BBa_J69801.1 FEATURES Location/Qualifiers misc_feature 1..129 /label=BBa_B0015 misc_feature 131..136 /label=SphI misc_feature 138..893 /label=C0362 luxR (codon-optimised) RBS 900..911 /label=B0034 misc_feature 913..918 /label=BamHI promoter 920..974 /label=R0011 - lac lamda CI hybrid misc_feature 976..981 /label=BspH promoter 983..1037 /label=R0062 - lux pR misc_feature 1..1037 /label=BBa_J69010 misc_feature 1050..1053 /label=conserved RBS 1046..1057 /label=BBa_B0034 CDS 1064..1783 /label=GFP protein CDS 1064..1783 /label=BBa_E0040 stem_loop 1803..1846 /label=stem_loop misc_feature 1792..1871 /label=BBa_B0010 terminator 1792..1871 /label=BBa_B0010 stem_loop 1887..1906 /label=T7 TE misc_feature 1913..1913 /label=stop polyA_site 1907..1920 /label=polya misc_feature 1880..1920 /label=BBa_B0012 terminator 1880..1920 /label=BBa_B0012 misc_feature 1..1920 /label=BBa_J69011 promoter 3008..3138 /label=Pbad promoter 2971..3042 /label=PC misc_feature 1929..3007 /label=AraC promoter 1929..3138 /label=BBa_I0500 misc_feature 3151..3154 /label=conserved RBS 3147..3158 /label=BBa_B0034 misc_feature 3165..3167 /label=start misc_feature 3948..3953 /label=2 misc_feature 3915..3947 /label=LVA CDS 3165..3914 /label=aiiA misc_feature 3165..3953 /label=BBa_C0060 misc_feature 3954..3978 /label=Help:Barcodes CDS 3165..3953 /label=BBa_C0060 misc_feature 3991..3994 /label=conserved RBS 3987..3998 /label=BBa_B0034 CDS 4005..4727 /label=BBa_E0020 stem_loop 4747..4790 /label=stem_loop misc_feature 4736..4815 /label=BBa_B0010 terminator 4736..4815 /label=BBa_B0010 stem_loop 4831..4850 /label=T7 TE misc_feature 4857..4857 /label=stop polyA_site 4851..4864 /label=polya misc_feature 4824..4864 /label=BBa_B0012 terminator 4824..4864 /label=BBa_B0012 misc_feature 1929..4864 /label=BBa_I0403 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt gcatgcgtta ttagtttttg aaatacgggc aatcgatcgc accggtcagg 181 atagctttgc tgatagattg acaacgattc gtggtgttca gtttcatctg cgcgttggtc 241 agatggaaag ttacggtgcg ttcagagcaa cccagaattt tggagatgtc ccaagaggac 301 ttaccttcgc acgcccatgc caggcattct ttctcgcgtt tggtcaggtc gttgttgctt 361 ttgttattgg caatgttaat cttgcgatag ttatcaacca gggacggaac gatcagagga 421 atgttcatgc acgcgtgcag gaacagagaa tcgatgtagt tgtctttttc ggagtgcgca 481 aaagacagca taccgaagcc gttgttcgcg gtatgaatcg ggaagctgaa accggtgatc 541 agaccgctgg tttttgcttc tttgataacg tttggggact ttttgttaac cgcgttgttc 601 tcaaagatat tccagttaat cggggaatgg ttagagttag agtaatctac gatcggatcg 661 tacttgatca ggttggcgtc atcatagtac tgacgccatt tttttgggta gttatccaga 721 atagaaatat cggattttac catgctgtgc ggataaatga tggccagcag ataatattca 781 cagtgaacca ttttggtcat atcagacagg cattggttaa tatcgttgtt agaacgacac 841 gctttgattt tgttaatgat acggtaggtg tcgtccgcgt tgatgttttt catctagtat 901 ttctcctctt ttggatccgt gtgctcagta tcttgttatc cgctcacaat gtcaattgtt 961 atccgctcac aattttcatg agacctgtag gatcgtacag gtttacgcaa gaaaatggtt 1021 tgttatagtc gaataaatac tagagaaaga ggagaaatac tagatgcgta aaggagaaga 1081 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 1141 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 1201 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta 1261 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag 1321 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta 1381 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa 1441 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa 1501 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa 1561 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac 1621 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc 1681 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc 1741 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc 1801 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1861 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1921 tactagagtt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg 1981 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg 2041 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc 2101 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg 2161 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata 2221 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta 2281 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca 2341 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt 2401 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta 2461 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa 2521 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct 2581 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc 2641 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg 2701 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca 2761 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc 2821 ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc 2881 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa 2941 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga 3001 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc 3061 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac 3121 ccgttttttt gggctagcta ctagagaaag aggagaaata ctagatgaca gtaaagaagc 3181 tttatttcgt cccagcaggt cgttgtatgt tggatcattc gtctgttaat agtacattaa 3241 caccaggaga attattagac ttaccggttt ggtgttatct tttggagact gaagaaggac 3301 ctattttagt agatacaggt atgccagaaa gtgcagttaa taatgaaggt ctttttaacg 3361 gtacatttgt cgaagggcag gttttaccga aaatgactga agaagataga atcgtgaata 3421 ttttaaaacg ggttggttat gagccggaag accttcttta tattattagt tctcacttgc 3481 attttgatca tgcaggagga aatggcgctt ttataaatac accaatcatt gtacagcgtg 3541 ctgaatatga ggcggcgcag catagcgaag aatatttgaa agaatgtata ttgccgaatt 3601 taaactacaa aatcattgaa ggtgattatg aagtcgtacc aggagttcaa ttattgcata 3661 caccaggcca tactccaggg catcaatcgc tattaattga gacagaaaaa tccggtcctg 3721 tattattaac gattgatgca tcgtatacga aagagaattt tgaaaatgaa gtgccatttg 3781 cgggatttga ttcagaatta gctttatctt caattaaacg tttaaaagaa gtggtgatga 3841 aagagaagcc gattgttttc tttggacatg atatagagca ggaaagggga tgtaaagtgt 3901 tccctgaata tatagctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat 3961 agtgctagtg tagatcgcta ctagagaaag aggagaaata ctagatggtg agcaagggcg 4021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtgaacggcc 4081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 4141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 4201 cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 4261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 4321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 4381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 4441 acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact 4501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 4561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 4621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 4681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac tagagccagg 4741 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 4801 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 4861 tata //