LOCUS       BBa_J69999              1880 bp    DNA     linear       29-Nov-2024
DEFINITION  Temp
ACCESSION   BBa_J69999
VERSION     BBa_J69999.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..798
                     /label=BBa_E0030
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
     terminator      807..935
                     /label=BBa_B0015
     misc_feature    1..935
                     /label=BBa_J69504
     misc_feature    944..962
                     /label=TetR 1
     misc_feature    944..997
                     /label=BBa_R0040
     promoter        963..968
                     /label=-35
     misc_feature    969..987
                     /label=TetR 2
     promoter        986..991
                     /label=-10
     promoter        944..997
                     /label=BBa_R0040
     misc_feature    1010..1013
                     /label=conserved
     RBS             1006..1017
                     /label=BBa_B0034
     CDS             1024..1743
                     /label=GFP protein
     CDS             1024..1743
                     /label=BBa_E0040
     stem_loop       1763..1806
                     /label=stem_loop
     misc_feature    1752..1831
                     /label=BBa_B0010
     terminator      1752..1831
                     /label=BBa_B0010
     stem_loop       1847..1866
                     /label=T7 TE
     misc_feature    1873..1873
                     /label=stop
     polyA_site      1867..1880
                     /label=polya
     misc_feature    1840..1880
                     /label=BBa_B0012
     terminator      1840..1880
                     /label=BBa_B0012
     misc_feature    944..1880
                     /label=BBa_J69521
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac
      301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
      601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga
      961 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac
     1021 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
     1081 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
     1141 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
     1201 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
     1261 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
     1321 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
     1381 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
     1441 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
     1501 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
     1561 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
     1621 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
     1681 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     1741 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1801 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1861 tgggcctttc tgcgtttata
//