BBa_J70007 1 BBa_J70007 recT recombination gene from Spiroplasma citri 2008-04-07T11:00:00Z 2015-05-08T01:08:20Z Genbank entry CAK99381 protein, nucleotide coding region. Mutations of the single GATC site to avoid restriction by the Sau3AI restriction system in Me. florum. PCR from S. citri DNA, kind gift of Prof. J. Renaudin. recET recombination gene from Spiroplasma citri, probably catalyzing the homologous recombination of DNA fragments similar to the Lambda Bet gene (Lambda RED recombination). This gene is from S. citri, a close relative of Me. florum, and has very similar codon usage. false false _41_48_1_ 0 6 48 Not in stock false Eliminate GATC site by silent mutation to GACC (asp -> asp). Note very low GC content at the 5' end. The primers are: * gtttcttcgaattcgcggccgcttctagatgaataataaaattgaattaatattaaataattc,recT-F * gtttcttctactagtagcggccgctgcagttattaatcagaaaacatttcattaacagc,recT-R * gaaatgcaagaagcctatcaacatgaccaagcaataattattaataacagc,recT-mut-F * gctgttattaataattattgcttggtcatgttgataggcttcttgcatttc,recT-mut-R false Tom Knight annotation1983023 1 recT range1983023 1 1 858 BBa_J70007_sequence 1 atgaataataaaattgaattaatattaaataattcaaaagttaataattgaattaatgaaaaattaacaaatcagaaagaaattaatttatttcgtaaaaatatcttaacaatttataataataatcccaatttatttgataaagaaaaaataaatttaatgagtgttttatctgcttgtgtaaaagcaatgttattagatttaccgttagaccctaacttaggatatgcctatattgttccatataatggaaaaggacagttgcaaattggttataagggttatattcagttagcaatccgaacaggaaaatatttaacaataaacgcaatagaagtaaaacaaggtgaattattaaattttgatgaattagaacaagaatacaattttaaatgaataaccaacgaaaatgaacgaacaaaaaaagaaacaatcggatatgttgcattttttaaactactaaatggttataaaaaaactttatattgaagtaaagaaaaatgcgaaaatcattttaaaacatattctaagaattatcaaacttacggaaaatttacggctggaagttatgaggggatggcacttaaaacagtattaacccaacttttaagaaaatgaggtattatgtcagttgaaatgcaagaagcctatcaacatgaccaagcaataattattaataacagcaaagagtttagtgataatcctaacataaaaaataaagaagataacaatgttattgaattaaataacaaaaaagatgaattaaatctaaatttagaacaagaatttagcagtaatgaaataaatgatgatgaagaaattgctaaagctgttaatgaaatgttttctgattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z