LOCUS       BBa_J70022              2978 bp    DNA     linear       22-Dec-2024
DEFINITION  GENEART pGA4 plasmid
ACCESSION   BBa_J70022
VERSION     BBa_J70022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=BioBrick suffix
     misc_feature    2958..2978
                     /label=BioBrick prefix
     promoter        107..126
                     /label=T3 promoter
     promoter        196..225
                     /label=lac promoter
     misc_feature    534..1153
                     /label=pBR322 origin
     CDS             1308..2168
                     /label=beta-lactamase ampicillin resistance marker
     promoter        2210..2238
                     /label=beta-lactamase (ampR) promoter
     misc_feature    2431..2737
                     /label=f1 origin
     CDS             2755..2914
                     /label=lacZalpha reporter
     promoter        2922..2940
                     /label=T7 promoter
     misc_feature    567..1007
                     /label=ColE1 origin
     misc_feature    2299..2755
                     /label=f1 (+) origin
     misc_feature    2796..2954
                     /label=D segment
ORIGIN
        1 actagtagcg gccgctgcag gctaagcttg atatcgaatt cctgcagccc gggggatccg
       61 cccgggctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
      121 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      181 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      241 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      301 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      361 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1441 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2281 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     2341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc tatggcccac
     2521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     2761 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2821 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2881 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     2941 gcgaattggg taccgcagaa ttcgcggccg cttctaga
//