LOCUS       BBa_J70023              1877 bp    DNA     linear       27-Dec-2024
DEFINITION  MF-plasmid-ori
ACCESSION   BBa_J70023
VERSION     BBa_J70023.1  
FEATURES             Location/Qualifiers
     primer_bind     1..27
                     /label=forward PCR priimer
     primer_bind     177..201
                     /label=fwd2
     misc_feature    328..336
                     /label=dnaA binding
     misc_feature    394..402
                     /label=dnaA binding
     misc_feature    458..469
                     /label=imperfect repeat
     misc_feature    472..480
                     /label=dnaA binding
     misc_feature    579..589
                     /label=imperfect repeat
     misc_feature    763..771
                     /label=dnaA binding
     RBS             816..820
                     /label=rbs
     CDS             830..1483
                     /label=pe
     misc_feature    1481..1491
                     /label=Iteron
     misc_feature    1501..1511
                     /label=Iteron
     misc_feature    1646..1822
                     /label=Low GC region
     primer_bind     1854..1877
                     /label=reverse PCR primer
ORIGIN
        1 gccctacttg tttagatagt ttaattcaac atttaaataa gtaataaatt atgttaaaat
       61 ttacttagac acttatttat aaattccttt taaataaaag gtagcggttt aaagtgtcat
      121 aatcatttat tttgttttat tttttttatt aattcttgta atgtatttaa atataataat
      181 tataaaaaca ccttatttta ctaaggtgtt ttatttttta acaaaatatt tgataaattt
      241 atttatctgt tatacaatgt aattgttagc aattaaattg ctaggacaat taaaaacgga
      301 aacacaatgc aatttgaaga caactgtgtt tccataaaca tcttaacata ttttatttaa
      361 cttagaatgt gttatatcaa ttattataat gtttgtggaa atagcggtct gttatttccc
      421 tttttatttg ttacaaaaaa ggagttgaaa ttaaattatt taatccccct ttgtggtaga
      481 ataaaaacaa ctaaatagaa acaaagaaaa atgactccca cgtcattctt ctatacacct
      541 attaagcggt ctcacaaacc acttaactag ggttcttaat ttaaccccca ccttttttgg
      601 taggtcagtt aaattataac attttttaat ttttgtgtat aggtagagtg ataaaaaagt
      661 cattctacct atttttttta tttaatatat caaaacaaca aaaaaatcag tttatatcag
      721 cgttgaaata accgaaaaga atgaagagaa gatttattac attttggaaa atatcaacgc
      781 tgatatgggg tttaaattcg ctcaaaaccg tagaaaggag catattatta tgcaacatca
      841 acaacaaaca gaacaactca attatcactt tgttgagata aatgggttga gttataaagt
      901 gattaatcaa cttgacgaga aaaaacatat ctctaatttt tacttaccga aaaaaattgt
      961 tcgccaacac cctacccgcc aagacagtta taaagtcaaa atttatgaca aatttatttg
     1021 tgttcctaaa aaaatgtgtt tcttagacaa aactggtaaa tactttttgg ttggtcttga
     1081 tatgtttttt aactactgaa tttataatac acgagataat aacaaatatc gcttgacggg
     1141 ttatcaagcg attaaagata ctgcaattaa agaattgcat tatttaactg tgtctgctcg
     1201 ccgctacgaa aaagaacaag caacaaaccc ttttttaagt ggattaactt accaacaagc
     1261 acgcgaccaa atttgtgctg aaagcgacaa actaaaagca gaataccaat ctttcgtaaa
     1321 aactaagtat tctgctaaaa acagtgaaaa tttacttacc agagaaaata attctcttgt
     1381 tgatgattat aacactgttt atgaaaaaaa caagggagaa ccaccagaaa tgtccgaaga
     1441 agaactcact gagttattaa cggaattaga caacttagac taactcccct atcgagttct
     1501 taactcccct accaacaaag cacagaactt gcgtaaaacc acagcgtagc aagacaatga
     1561 atataatact aagatatggt ctttatcata tcgtttttgc tgtttagtaa aaagcgatat
     1621 tttttatttc tttttctccc aatagaaaac caaaataaat accattacta tatcaaaaca
     1681 aaatatttaa taaattttat atactttttt aagctactat ttagattatt ttatttaaaa
     1741 agtactaaat atattaaaat ttaagatata atttatatag taatataata gagaataata
     1801 agtatttaca ttttttatta tactgcggaa caaaaagcat tttggcacgc caagcacccc
     1861 aaaccccgca ctcccaa
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