BBa_J70012
1
BBa_J70012
A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page
2008-05-08T11:00:00Z
2015-05-08T01:08:20Z
designed
still in testing
false
false
_41_
0
1201
41
Not in stock
false
still in testing
false
Julie Norville
annotation1963016
1
PsrI 1
range1963016
1
10
12
annotation1963021
1
AscI 1
range1963021
1
20
27
annotation1963022
1
AscI 2
range1963022
1
28
34
annotation1963015
1
PsrI 1
range1963015
1
1
3
annotation1963017
1
PsrI 2
range1963017
1
43
46
annotation1963018
1
Mab 1
range1963018
1
4
10
annotation1963020
1
Mab 2
range1963020
1
47
52
BBa_J70030
1
BBa_J70030
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
2008-12-07T12:00:00Z
2015-05-08T01:08:20Z
this part is designed
When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scar
still in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector
false
false
_41_
0
1201
41
Not in stock
false
Can be used to removed protein tails for protein fusions, see BBF RFC 15
XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX XXX ATGATCAC ATGATCTA^XXXXX
XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX ATT ATGATCAC ATGATCTA^XXXXX
false
Julie Norville
annotation2000010
1
PacI 1
range2000010
1
14
20
annotation2000009
1
MabI 1
range2000009
1
8
14
annotation2000013
1
MabI 2
range2000013
1
30
36
annotation2000008
1
PpiI 1
range2000008
1
4
7
annotation2000012
1
PacI 2
range2000012
1
22
29
annotation2000011
1
PacI3
range2000011
1
18
24
annotation2000014
1
PpiI 2
range2000014
1
37
40
annotation2000015
1
PpiI 2
range2000015
1
46
48
BBa_J70031
1
BBa_J70031
Linker for BioScaffold Parts, example of Gamma BioScaffold part
2008-12-12T12:00:00Z
2015-05-08T01:08:20Z
designed
Will use as a linker when fusing two BioScaffold parts, can be created as an oligo
false
false
_41_
0
1201
41
Not in stock
false
needs to be longer than 28 bp
false
Julie Norville
annotation2000306
1
BBa Scar
range2000306
1
8
15
annotation2000304
1
MabI
range2000304
1
1
7
annotation2000305
1
MabI
range2000305
1
16
22
BBa_J70032
1
BBa_J70032
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)
2008-12-12T12:00:00Z
2015-05-08T01:08:20Z
Designed
Brings together a BioScaffold part that removes protein tails with one that removes protein heads in order to create a BioScaffold part that can help create protein fusions using standard assembly
false
false
_41_
0
1201
41
Not in stock
false
The part that links the two BioScaffold parts can be altered as desired--here I just wanted to leave at least 7 bp beyond the internal scars.
false
Julie Norville
component2000365
1
BBa_J70012
component2000353
1
BBa_J70030
component2000357
1
BBa_J70031
annotation2000357
1
BBa_J70031
range2000357
1
57
78
annotation2000353
1
BBa_J70030
range2000353
1
1
48
annotation2000365
1
BBa_J70012
range2000365
1
87
138
BBa_J70032_sequence
1
ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctctactagagaccaggttactagagacctggttactagagaacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccagg
BBa_J70031_sequence
1
accaggttactagagacctggt
BBa_J70030_sequence
1
ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctc
BBa_J70012_sequence
1
aacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccagg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z