LOCUS       BBa_J70037              1116 bp    DNA     linear       28-Jun-2024
DEFINITION  Library Vector Test Part
ACCESSION   BBa_J70037
VERSION     BBa_J70037.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    209..215
                     /label=AarI
     misc_feature    228..233
                     /label=XmaI
     misc_feature    246..252
                     /label=AarI
     misc_feature    209..253
                     /label=BBa_J70034
     CDS             260..979
                     /label=GFP protein
     misc_feature    260..979
                     /label=BBa_E0040
     misc_feature    988..1067
                     /label=BBa_B0010
     stem_loop       999..1042
                     /label=stem_loop
     misc_feature    1076..1116
                     /label=BBa_B0012
     stem_loop       1083..1102
                     /label=T7 TE
     polyA_site      1103..1116
                     /label=polya
     misc_feature    1109..1109
                     /label=stop
     misc_feature    201..204
                     /label=5' overhang
     misc_feature    257..260
                     /label=3' overhang
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggc aggtggacaa gaggagtccc gggagctgga
      241 actcccacct gcatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      301 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      361 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      421 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      481 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      541 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      601 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      661 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      721 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      781 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      841 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      901 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      961 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1021 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1081 ctggctcacc ttcgggtggg cctttctgcg tttata
//