BBa_J70060
1
BBa_J70060
BBa_J70010 (PpiI) protein fusion test part
2009-02-05T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This is used to test whether J70010 (PpiI) can be used to make protein fusions.
false
false
_41_
0
1201
41
Not in stock
false
Needed to line up the gly ser on each side of the BioScaffold part properly so that they would anneal after the BioScaffold part was removed.
false
Julie Norville
component2000286
1
BBa_J70044
component2000271
1
BBa_J70042
component2000281
1
BBa_J70010
annotation2000286
1
BBa_J70044
range2000286
1
97
122
annotation2000271
1
BBa_J70042
range2000271
1
1
27
annotation2000281
1
BBa_J70010
range2000281
1
36
88
BBa_J70042
1
BBa_J70042
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein.
This part is still in testing.
During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.
false
Julie Norville
annotation2000133
1
gly-ser
range2000133
1
22
27
annotation2000132
1
6 his
range2000132
1
4
21
annotation2000131
1
Start
range2000131
1
1
3
annotation2000134
1
N terminal 6 his
range2000134
1
1
27
BBa_J70010
1
BBa_J70010
A BioScaffold Part (Uses PpiI), for offset see Part Design page
2008-05-08T11:00:00Z
2015-05-08T01:08:20Z
designed
still in testing
false
false
_41_
0
1201
41
Not in stock
false
still in testing
false
Julie Norville
annotation1963028
1
PpiI 2
range1963028
1
42
45
annotation1963030
1
MabI 1
range1963030
1
13
19
annotation1963033
1
PacI 2
range1963033
1
27
34
annotation1963034
1
PacI3
range1963034
1
23
30
annotation1963032
1
PacI 1
range1963032
1
19
24
annotation1963031
1
MabI 2
range1963031
1
35
41
annotation1963026
1
PpiI 1
range1963026
1
1
3
annotation1963029
1
PpiI2
range1963029
1
51
53
annotation1963027
1
PpiI 1
range1963027
1
9
12
BBa_J70044
1
BBa_J70044
6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
This 6 His suffix tag can be used with BBa_J70010 (PpiI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This is 6 his suffix tag is designed to be used with BBa_J70010 (PpiI) BioScaffold parts.
false
Julie Norville
annotation2000135
1
gly-ser
range2000135
1
1
5
annotation2000136
1
6 his
range2000136
1
6
23
annotation2000137
1
stop
range2000137
1
24
26
annotation2000138
1
C terminal 6 his
range2000138
1
1
26
BBa_J70010_sequence
1
gagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctc
BBa_J70042_sequence
1
atgcaccatcaccatcatcatggatcc
BBa_J70044_sequence
1
gatcccaccatcaccatcatcattaa
BBa_J70060_sequence
1
atgcaccatcaccatcatcatggatcctactagaggagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctctactagaggatcccaccatcaccatcatcattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z