BBa_J70044
1
BBa_J70044
6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
This 6 His suffix tag can be used with BBa_J70010 (PpiI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This is 6 his suffix tag is designed to be used with BBa_J70010 (PpiI) BioScaffold parts.
false
Julie Norville
annotation2000135
1
gly-ser
range2000135
1
1
5
annotation2000136
1
6 his
range2000136
1
6
23
annotation2000137
1
stop
range2000137
1
24
26
annotation2000138
1
C terminal 6 his
range2000138
1
1
26
BBa_J70010
1
BBa_J70010
A BioScaffold Part (Uses PpiI), for offset see Part Design page
2008-05-08T11:00:00Z
2015-05-08T01:08:20Z
designed
still in testing
false
false
_41_
0
1201
41
Not in stock
false
still in testing
false
Julie Norville
annotation1963030
1
MabI 1
range1963030
1
13
19
annotation1963033
1
PacI 2
range1963033
1
27
34
annotation1963027
1
PpiI 1
range1963027
1
9
12
annotation1963028
1
PpiI 2
range1963028
1
42
45
annotation1963031
1
MabI 2
range1963031
1
35
41
annotation1963032
1
PacI 1
range1963032
1
19
24
annotation1963034
1
PacI3
range1963034
1
23
30
annotation1963026
1
PpiI 1
range1963026
1
1
3
annotation1963029
1
PpiI2
range1963029
1
51
53
BBa_J70064
1
BBa_J70064
Adds 6 his suffix, using BioScaffold part J70010 (PpiI)
2009-02-06T12:00:00Z
2015-05-08T01:08:20Z
Designed
Created as an intermediate on the way to making J70060, but keeping it because it can be useful on its own for adding 6 his suffix to parts (replace part J70042 in J70060 with your part, adding the appropriate glycine serine suffix to you part.)
false
false
_41_
0
1201
41
Not in stock
false
Needs to line up in the right position so that when the BioScaffold part is removed, then the left and right part anneal.
false
Julie Norville
component2000380
1
BBa_J70044
component2000375
1
BBa_J70010
annotation2000380
1
BBa_J70044
range2000380
1
62
87
annotation2000375
1
BBa_J70010
range2000375
1
1
53
BBa_J70010_sequence
1
gagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctc
BBa_J70044_sequence
1
gatcccaccatcaccatcatcattaa
BBa_J70064_sequence
1
gagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctctactagaggatcccaccatcaccatcatcattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z