BBa_J70048
1
BBa_J70048
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA
2009-02-05T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 6 His suffix tag can be used with BBa_J70012 (PsrI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.
false
false
_41_
0
1201
41
Not in stock
false
Designed.
false
Julie Norville
annotation2000265
1
gly-ser
range2000265
1
4
9
annotation2000263
1
C terminal his tag
range2000263
1
1
30
annotation2000262
1
Start
range2000262
1
1
3
annotation2000266
1
stop
range2000266
1
28
30
annotation2000264
1
6 his
range2000264
1
10
27
BBa_J70070
1
BBa_J70070
BBa_J70012 (PsrI) protein fusion test part
2009-02-06T12:00:00Z
2015-05-08T01:08:20Z
Designed
This is used to test whether J70012 (PsrI) can be used to make protein fusions (can also be cut with BamHI.)
After excision <Prefix w/AT>G protein G GATCC^ ^ protein TAA<Suffix>
C^ ^CTAGG
false
false
_41_
0
1201
41
Not in stock
false
The glycine serine parts need to fit together.
false
Julie Norville
component2000393
1
BBa_J70012
component2000385
1
BBa_J70042
component2000399
1
BBa_J70048
annotation2000399
1
BBa_J70048
range2000399
1
94
123
annotation2000393
1
BBa_J70012
range2000393
1
36
87
annotation2000385
1
BBa_J70042
range2000385
1
1
27
BBa_J70012
1
BBa_J70012
A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page
2008-05-08T11:00:00Z
2015-05-08T01:08:20Z
designed
still in testing
false
false
_41_
0
1201
41
Not in stock
false
still in testing
false
Julie Norville
annotation1963018
1
Mab 1
range1963018
1
4
10
annotation1963022
1
AscI 2
range1963022
1
28
34
annotation1963015
1
PsrI 1
range1963015
1
1
3
annotation1963020
1
Mab 2
range1963020
1
47
52
annotation1963016
1
PsrI 1
range1963016
1
10
12
annotation1963021
1
AscI 1
range1963021
1
20
27
annotation1963017
1
PsrI 2
range1963017
1
43
46
BBa_J70042
1
BBa_J70042
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein.
This part is still in testing.
During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.
false
Julie Norville
annotation2000133
1
gly-ser
range2000133
1
22
27
annotation2000132
1
6 his
range2000132
1
4
21
annotation2000131
1
Start
range2000131
1
1
3
annotation2000134
1
N terminal 6 his
range2000134
1
1
27
BBa_J70042_sequence
1
atgcaccatcaccatcatcatggatcc
BBa_J70048_sequence
1
atgggatcccaccatcaccatcatcattaa
BBa_J70012_sequence
1
aacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccagg
BBa_J70070_sequence
1
atgcaccatcaccatcatcatggatcctactagagaacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccaggtactagatgggatcccaccatcaccatcatcattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z