BBa_J70030
1
BBa_J70030
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
2008-12-07T12:00:00Z
2015-05-08T01:08:20Z
this part is designed
When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scar
still in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector
false
false
_41_
0
1201
41
Not in stock
false
Can be used to removed protein tails for protein fusions, see BBF RFC 15
XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX XXX ATGATCAC ATGATCTA^XXXXX
XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX ATT ATGATCAC ATGATCTA^XXXXX
false
Julie Norville
annotation2000008
1
PpiI 1
range2000008
1
4
7
annotation2000009
1
MabI 1
range2000009
1
8
14
annotation2000010
1
PacI 1
range2000010
1
14
20
annotation2000013
1
MabI 2
range2000013
1
30
36
annotation2000012
1
PacI 2
range2000012
1
22
29
annotation2000011
1
PacI3
range2000011
1
18
24
annotation2000015
1
PpiI 2
range2000015
1
46
48
annotation2000014
1
PpiI 2
range2000014
1
37
40
BBa_J70044
1
BBa_J70044
6his part for use with BioScaffold parts (G)GATCC(glycine serine)-6his-TAA
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
This 6 His suffix tag can be used with BBa_J70010 (PpiI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This is 6 his suffix tag is designed to be used with BBa_J70010 (PpiI) BioScaffold parts.
false
Julie Norville
annotation2000135
1
gly-ser
range2000135
1
1
5
annotation2000136
1
6 his
range2000136
1
6
23
annotation2000137
1
stop
range2000137
1
24
26
annotation2000138
1
C terminal 6 his
range2000138
1
1
26
BBa_J70080
1
BBa_J70080
BBa_J70030 (PpiI) protein fusion test part
2009-02-06T12:00:00Z
2015-05-08T01:08:20Z
Designed
This is used to test whether J70030 (PpiI) can be used to make protein fusions (can also be cut with BamHI.)
false
false
_41_
0
1201
41
Not in stock
false
Make sure the glycine serine portions come together.
false
Julie Norville
component2000414
1
BBa_J70030
component2000419
1
BBa_J70044
component2000405
1
BBa_J70046
annotation2000414
1
BBa_J70030
range2000414
1
39
86
annotation2000405
1
BBa_J70046
range2000405
1
1
30
annotation2000419
1
BBa_J70044
range2000419
1
95
120
BBa_J70046
1
BBa_J70046
6his part for use with BioScaffold parts ATG-6 his-GATCC(glycine serine)-TAA
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 6 His prefix tag can be used with BBa_J70030 (PpiI) in fusing a 6 his tag to the N-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
It was designed to work with BBa_J70030
false
Julie Norville
annotation2000142
1
Stop
range2000142
1
28
30
annotation2000141
1
dna
range2000141
1
22
26
annotation2000139
1
Start
range2000139
1
1
3
annotation2000140
1
6 his
range2000140
1
4
21
annotation2000143
1
6 his N-terminal tag
range2000143
1
1
30
BBa_J70044_sequence
1
gatcccaccatcaccatcatcattaa
BBa_J70030_sequence
1
ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctc
BBa_J70080_sequence
1
atgcaccatcaccatcatcatggatcctaatactagagctggttcacctggttaattaattaattaaaccaggtgaacgtggtctctactagaggatcccaccatcaccatcatcattaa
BBa_J70046_sequence
1
atgcaccatcaccatcatcatggatcctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z