LOCUS BBa_J70090 209 bp DNA linear 22-Nov-2024 DEFINITION BBa_J70032 (PpiI/PsrI) protein fusion test part ACCESSION BBa_J70090 VERSION BBa_J70090.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=Start CDS 4..21 /label=6 his misc_feature 22..27 /label=gly-ser misc_feature 1..27 /label=N terminal 6 his misc_feature 1..27 /label=BBa_J70042 misc_feature 39..42 /label=PpiI 1 misc_feature 43..49 /label=MabI 1 misc_feature 49..55 /label=PacI 1 misc_feature 53..59 /label=PacI3 misc_feature 57..64 /label=PacI 2 misc_feature 65..71 /label=MabI 2 misc_feature 72..75 /label=PpiI 2 misc_feature 81..83 /label=PpiI 2 misc_feature 36..83 /label=BBa_J70030 misc_feature 92..98 /label=MabI misc_feature 107..113 /label=MabI misc_feature 99..106 /label=BBa Scar misc_feature 92..113 /label=BBa_J70031 misc_feature 122..124 /label=PsrI 1 misc_feature 131..133 /label=PsrI 1 misc_feature 164..167 /label=PsrI 2 misc_feature 125..131 /label=Mab 1 misc_feature 168..173 /label=Mab 2 misc_feature 141..148 /label=AscI 1 misc_feature 149..155 /label=AscI 2 misc_feature 122..173 /label=BBa_J70012 misc_feature 180..182 /label=Start misc_feature 180..209 /label=C terminal his tag CDS 189..206 /label=6 his misc_feature 183..188 /label=gly-ser misc_feature 207..209 /label=stop misc_feature 180..209 /label=BBa_J70048 ORIGIN 1 atgcaccatc accatcatca tggatcctac tagagctggt tcacctggtt aattaattaa 61 ttaaaccagg tgaacgtggt ctctactaga gaccaggtta ctagagacct ggttactaga 121 gaacacctgg tacagcaggt ggcgcgccgg cgcgccagct ggtgaacacc aggtactaga 181 tgggatccca ccatcaccat catcattaa //