##gff-version 3 ##sequence-region BBa_J70090 1 209 BBa_J70090 . tag 180 209 . + 0 ID=BBa_J70048;Name=BBa_J70048 BBa_J70090 . sequence_feature 183 188 . + 0 ID=annotation2000265;Name=gly-ser;Parent=BBa_J70048 BBa_J70090 . tag 180 209 . + 0 ID=annotation2000263;Name=C terminal his tag;Parent=BBa_J70048 BBa_J70090 . start_codon 180 182 . + 0 ID=annotation2000262;Name=Start;Parent=BBa_J70048 BBa_J70090 . stop_codon 207 209 . + 0 ID=annotation2000266;Name=stop;Parent=BBa_J70048 BBa_J70090 . CDS 189 206 . + 0 ID=annotation2000264;Name=6 his;Parent=BBa_J70048 BBa_J70090 . sequence_feature 36 83 . + 0 ID=BBa_J70030;Name=BBa_J70030 BBa_J70090 . sequence_feature 72 75 . + 0 ID=annotation2000014;Name=PpiI 2;Parent=BBa_J70030 BBa_J70090 . non_covalent_binding_site 57 64 . + 0 ID=annotation2000012;Name=PacI 2;Parent=BBa_J70030 BBa_J70090 . non_covalent_binding_site 53 59 . + 0 ID=annotation2000011;Name=PacI3;Parent=BBa_J70030 BBa_J70090 . sequence_feature 65 71 . + 0 ID=annotation2000013;Name=MabI 2;Parent=BBa_J70030 BBa_J70090 . sequence_feature 39 42 . + 0 ID=annotation2000008;Name=PpiI 1;Parent=BBa_J70030 BBa_J70090 . sequence_feature 81 83 . + 0 ID=annotation2000015;Name=PpiI 2;Parent=BBa_J70030 BBa_J70090 . non_covalent_binding_site 49 55 . + 0 ID=annotation2000010;Name=PacI 1;Parent=BBa_J70030 BBa_J70090 . sequence_feature 43 49 . + 0 ID=annotation2000009;Name=MabI 1;Parent=BBa_J70030 BBa_J70090 . sequence_feature 122 173 . + 0 ID=BBa_J70012;Name=BBa_J70012 BBa_J70090 . sequence_feature 168 173 . + 0 ID=annotation1963020;Name=Mab 2;Parent=BBa_J70012 BBa_J70090 . non_covalent_binding_site 141 148 . + 0 ID=annotation1963021;Name=AscI 1;Parent=BBa_J70012 BBa_J70090 . sequence_feature 125 131 . + 0 ID=annotation1963018;Name=Mab 1;Parent=BBa_J70012 BBa_J70090 . sequence_feature 122 124 . + 0 ID=annotation1963015;Name=PsrI 1;Parent=BBa_J70012 BBa_J70090 . sequence_feature 164 167 . + 0 ID=annotation1963017;Name=PsrI 2;Parent=BBa_J70012 BBa_J70090 . non_covalent_binding_site 149 155 . + 0 ID=annotation1963022;Name=AscI 2 ;Parent=BBa_J70012 BBa_J70090 . sequence_feature 131 133 . + 0 ID=annotation1963016;Name=PsrI 1;Parent=BBa_J70012 BBa_J70090 . sequence_feature 92 113 . + 0 ID=BBa_J70031;Name=BBa_J70031 BBa_J70090 . tag 99 106 . + 0 ID=annotation2000306;Name=BBa Scar;Parent=BBa_J70031 BBa_J70090 . sequence_feature 107 113 . + 0 ID=annotation2000305;Name=MabI;Parent=BBa_J70031 BBa_J70090 . sequence_feature 92 98 . + 0 ID=annotation2000304;Name=MabI;Parent=BBa_J70031 BBa_J70090 . tag 1 27 . + 0 ID=BBa_J70042;Name=BBa_J70042 BBa_J70090 . CDS 4 21 . + 0 ID=annotation2000132;Name=6 his;Parent=BBa_J70042 BBa_J70090 . start_codon 1 3 . + 0 ID=annotation2000131;Name=Start;Parent=BBa_J70042 BBa_J70090 . tag 1 27 . + 0 ID=annotation2000134;Name=N terminal 6 his;Parent=BBa_J70042 BBa_J70090 . sequence_feature 22 27 . + 0 ID=annotation2000133;Name=gly-ser;Parent=BBa_J70042 >BBa_J70090 atgcaccatcaccatcatcatggatcctactagagctggttcacctggttaattaattaattaaaccaggtgaacgtgg tctctactagagaccaggttactagagacctggttactagagaacacctggtacagcaggtggcgcgccggcgcgccag ctggtgaacaccaggtactagatgggatcccaccatcaccatcatcattaa