BBa_J70090
1
BBa_J70090
BBa_J70032 (PpiI/PsrI) protein fusion test part
2009-02-06T12:00:00Z
2015-05-08T01:08:20Z
Designed
This is used to test whether the protein fusion Bioscaffold part (BBa_J70032, PpiI and PsrI) can be used to make protein fusions (can also be cut with BamHI) from BioBricks with normal heads and tails.
Note that J70032 is a composite part made up of J70030, J70031, and J70012.
false
false
_41_
0
1201
41
Not in stock
false
Location of protein heads and tails and location of PpiI and PsrI cut sites.
false
Julie Norville
component2000424
1
BBa_J70042
component2000437
1
BBa_J70031
component2000445
1
BBa_J70012
component2000433
1
BBa_J70030
component2000451
1
BBa_J70048
annotation2000445
1
BBa_J70012
range2000445
1
122
173
annotation2000451
1
BBa_J70048
range2000451
1
180
209
annotation2000437
1
BBa_J70031
range2000437
1
92
113
annotation2000433
1
BBa_J70030
range2000433
1
36
83
annotation2000424
1
BBa_J70042
range2000424
1
1
27
BBa_J70031
1
BBa_J70031
Linker for BioScaffold Parts, example of Gamma BioScaffold part
2008-12-12T12:00:00Z
2015-05-08T01:08:20Z
designed
Will use as a linker when fusing two BioScaffold parts, can be created as an oligo
false
false
_41_
0
1201
41
Not in stock
false
needs to be longer than 28 bp
false
Julie Norville
annotation2000304
1
MabI
range2000304
1
1
7
annotation2000305
1
MabI
range2000305
1
16
22
annotation2000306
1
BBa Scar
range2000306
1
8
15
BBa_J70012
1
BBa_J70012
A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page
2008-05-08T11:00:00Z
2015-05-08T01:08:20Z
designed
still in testing
false
false
_41_
0
1201
41
Not in stock
false
still in testing
false
Julie Norville
annotation1963022
1
AscI 2
range1963022
1
28
34
annotation1963021
1
AscI 1
range1963021
1
20
27
annotation1963018
1
Mab 1
range1963018
1
4
10
annotation1963017
1
PsrI 2
range1963017
1
43
46
annotation1963016
1
PsrI 1
range1963016
1
10
12
annotation1963020
1
Mab 2
range1963020
1
47
52
annotation1963015
1
PsrI 1
range1963015
1
1
3
BBa_J70042
1
BBa_J70042
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein.
This part is still in testing.
During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.
false
Julie Norville
annotation2000132
1
6 his
range2000132
1
4
21
annotation2000133
1
gly-ser
range2000133
1
22
27
annotation2000131
1
Start
range2000131
1
1
3
annotation2000134
1
N terminal 6 his
range2000134
1
1
27
BBa_J70048
1
BBa_J70048
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA
2009-02-05T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 6 His suffix tag can be used with BBa_J70012 (PsrI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.
false
false
_41_
0
1201
41
Not in stock
false
Designed.
false
Julie Norville
annotation2000265
1
gly-ser
range2000265
1
4
9
annotation2000264
1
6 his
range2000264
1
10
27
annotation2000266
1
stop
range2000266
1
28
30
annotation2000262
1
Start
range2000262
1
1
3
annotation2000263
1
C terminal his tag
range2000263
1
1
30
BBa_J70030
1
BBa_J70030
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
2008-12-07T12:00:00Z
2015-05-08T01:08:20Z
this part is designed
When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scar
still in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector
false
false
_41_
0
1201
41
Not in stock
false
Can be used to removed protein tails for protein fusions, see BBF RFC 15
XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX XXX ATGATCAC ATGATCTA^XXXXX
XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^
^XXXXX ATT ATGATCAC ATGATCTA^XXXXX
false
Julie Norville
annotation2000010
1
PacI 1
range2000010
1
14
20
annotation2000009
1
MabI 1
range2000009
1
8
14
annotation2000013
1
MabI 2
range2000013
1
30
36
annotation2000011
1
PacI3
range2000011
1
18
24
annotation2000008
1
PpiI 1
range2000008
1
4
7
annotation2000015
1
PpiI 2
range2000015
1
46
48
annotation2000014
1
PpiI 2
range2000014
1
37
40
annotation2000012
1
PacI 2
range2000012
1
22
29
BBa_J70090_sequence
1
atgcaccatcaccatcatcatggatcctactagagctggttcacctggttaattaattaattaaaccaggtgaacgtggtctctactagagaccaggttactagagacctggttactagagaacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccaggtactagatgggatcccaccatcaccatcatcattaa
BBa_J70042_sequence
1
atgcaccatcaccatcatcatggatcc
BBa_J70031_sequence
1
accaggttactagagacctggt
BBa_J70048_sequence
1
atgggatcccaccatcaccatcatcattaa
BBa_J70030_sequence
1
ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctc
BBa_J70012_sequence
1
aacacctggtacagcaggtggcgcgccggcgcgccagctggtgaacaccagg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z