BBa_J70032 (PpiI/PsrI) protein fusion test part
This is used to test whether the protein fusion Bioscaffold part (BBa_J70032, PpiI and PsrI) can be used to make protein fusions (can also be cut with BamHI) from BioBricks with normal heads and tails. Note that J70032 is a composite part made up of J70030, J70031, and J70012.
Location of protein heads and tails and location of PpiI and PsrI cut sites.
A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page
When placed in a BioBrick assembly and cut with the restriction enzyme PpiI, this part will direct the restriction enzymes to cut outside the BioBrick scar still in testing, uses restriction enzyme sites PpiI (2) PacI (2) MabI (2), will place in pSB1AK3 vector
Can be used to removed protein tails for protein fusions, see BBF RFC 15 XXXXX^ XXX TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^ ^XXXXX XXX ATGATCAC ATGATCTA^XXXXX XXXXX^ TAA TACTAGAG BioScaffold Part BBa_J70030 TACTAGAT XXXXX^ ^XXXXX ATT ATGATCAC ATGATCTA^XXXXX
Linker for BioScaffold Parts, example of Gamma BioScaffold part
Will use as a linker when fusing two BioScaffold parts, can be created as an oligo
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K3.
This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA
This 6 His suffix tag can be used with BBa_J70012 (PsrI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.
Conversion of the iGEM parts registry to SBOL2.1