##gff-version 3 ##sequence-region BBa_J70100 1 116 BBa_J70100 . tag 1 27 . + 0 ID=BBa_J70042;Name=BBa_J70042 BBa_J70100 . CDS 4 21 . + 0 ID=annotation2000132;Name=6 his;Parent=BBa_J70042 BBa_J70100 . sequence_feature 22 27 . + 0 ID=annotation2000133;Name=gly-ser;Parent=BBa_J70042 BBa_J70100 . start_codon 1 3 . + 0 ID=annotation2000131;Name=Start;Parent=BBa_J70042 BBa_J70100 . tag 1 27 . + 0 ID=annotation2000134;Name=N terminal 6 his;Parent=BBa_J70042 BBa_J70100 . tag 87 116 . + 0 ID=BBa_J70048;Name=BBa_J70048 BBa_J70100 . sequence_feature 90 95 . + 0 ID=annotation2000265;Name=gly-ser;Parent=BBa_J70048 BBa_J70100 . stop_codon 114 116 . + 0 ID=annotation2000266;Name=stop;Parent=BBa_J70048 BBa_J70100 . start_codon 87 89 . + 0 ID=annotation2000262;Name=Start;Parent=BBa_J70048 BBa_J70100 . CDS 96 113 . + 0 ID=annotation2000264;Name=6 his;Parent=BBa_J70048 BBa_J70100 . tag 87 116 . + 0 ID=annotation2000263;Name=C terminal his tag;Parent=BBa_J70048 BBa_J70100 . sequence_feature 36 80 . + 0 ID=BBa_J70040;Name=BBa_J70040 BBa_J70100 . sequence_feature 64 69 . + 0 ID=annotation2000130;Name=MabI ;Parent=BBa_J70040 BBa_J70100 . non_covalent_binding_site 55 60 . + 0 ID=annotation2000128;Name=XmaI;Parent=BBa_J70040 BBa_J70100 . sequence_feature 36 42 . + 0 ID=annotation2000126;Name=AarI ;Parent=BBa_J70040 BBa_J70100 . sequence_feature 73 79 . + 0 ID=annotation2000127;Name=AarI ;Parent=BBa_J70040 BBa_J70100 . sequence_feature 46 52 . + 0 ID=annotation2000129;Name=MabI ;Parent=BBa_J70040 >BBa_J70100 atgcaccatcaccatcatcatggatcctactagaggcaggtggacacctagggtcccgggagcaccaggtcccacctgc atactagatgggatcccaccatcaccatcatcattaa