BBa_J70040
1
BBa_J70040
A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar
still in testing, uses AarI (2), MabI(2), XmaI (1) * XmaI does not work in vector for BioScaffold part manipulations
See part design page or BBFRFC15, for details about use
false
false
_41_
0
1201
41
Not in stock
false
See BBa_J70034, this is an analogous part except it uses MabI
false
Julie Norville
annotation2000128
1
XmaI
range2000128
1
20
25
annotation2000126
1
AarI
range2000126
1
1
7
annotation2000129
1
MabI
range2000129
1
11
17
annotation2000127
1
AarI
range2000127
1
38
44
annotation2000130
1
MabI
range2000130
1
29
34
BBa_J70042
1
BBa_J70042
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein.
This part is still in testing.
During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.
false
Julie Norville
annotation2000133
1
gly-ser
range2000133
1
22
27
annotation2000131
1
Start
range2000131
1
1
3
annotation2000132
1
6 his
range2000132
1
4
21
annotation2000134
1
N terminal 6 his
range2000134
1
1
27
BBa_J70102
1
BBa_J70102
Left 6 his test part for library construction part.
2009-03-01T12:00:00Z
2015-05-08T01:08:20Z
Testing
Testing
false
false
_41_
0
1201
41
Not in stock
false
Testing
false
Julie Norville
component2001775
1
BBa_J70042
component2001781
1
BBa_J70040
annotation2001775
1
BBa_J70042
range2001775
1
1
27
annotation2001781
1
BBa_J70040
range2001781
1
36
80
BBa_J70042_sequence
1
atgcaccatcaccatcatcatggatcc
BBa_J70102_sequence
1
atgcaccatcaccatcatcatggatcctactagaggcaggtggacacctagggtcccgggagcaccaggtcccacctgca
BBa_J70040_sequence
1
gcaggtggacacctagggtcccgggagcaccaggtcccacctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z