BBa_J70104
1
BBa_J70104
Right 6 his test part for library construction part.
2009-03-01T12:00:00Z
2015-05-08T01:08:20Z
Testing
Testing
false
false
_41_
0
1201
41
Not in stock
false
Testing
false
Julie Norville
component2001793
1
BBa_J70048
component2001787
1
BBa_J70040
annotation2001793
1
BBa_J70048
range2001793
1
52
81
annotation2001787
1
BBa_J70040
range2001787
1
1
45
BBa_J70040
1
BBa_J70040
A BioScaffold Part (Uses AarI, MabI) for Library Vector Preparation (see Part Design page)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar
still in testing, uses AarI (2), MabI(2), XmaI (1) * XmaI does not work in vector for BioScaffold part manipulations
See part design page or BBFRFC15, for details about use
false
false
_41_
0
1201
41
Not in stock
false
See BBa_J70034, this is an analogous part except it uses MabI
false
Julie Norville
annotation2000128
1
XmaI
range2000128
1
20
25
annotation2000126
1
AarI
range2000126
1
1
7
annotation2000129
1
MabI
range2000129
1
11
17
annotation2000127
1
AarI
range2000127
1
38
44
annotation2000130
1
MabI
range2000130
1
29
34
BBa_J70048
1
BBa_J70048
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA
2009-02-05T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 6 His suffix tag can be used with BBa_J70012 (PsrI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.
false
false
_41_
0
1201
41
Not in stock
false
Designed.
false
Julie Norville
annotation2000266
1
stop
range2000266
1
28
30
annotation2000264
1
6 his
range2000264
1
10
27
annotation2000263
1
C terminal his tag
range2000263
1
1
30
annotation2000265
1
gly-ser
range2000265
1
4
9
annotation2000262
1
Start
range2000262
1
1
3
BBa_J70104_sequence
1
gcaggtggacacctagggtcccgggagcaccaggtcccacctgcatactagatgggatcccaccatcaccatcatcattaa
BBa_J70048_sequence
1
atgggatcccaccatcaccatcatcattaa
BBa_J70040_sequence
1
gcaggtggacacctagggtcccgggagcaccaggtcccacctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z