BBa_J70042
1
BBa_J70042
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 7 His prefix tag and be used with BBa_J70010 (PpiI) and BBa_J70012 (PsrI) in fusing a 6 his tag to the N-terminus of a protein.
This part is still in testing.
During the BioScaffold part excision, it should be placed in pSB2K3.
false
false
_41_
0
1201
41
Not in stock
false
This can be used when creating standard fusions between the 6 his tag and protein parts using BioScaffold parts.
false
Julie Norville
annotation2000132
1
6 his
range2000132
1
4
21
annotation2000131
1
Start
range2000131
1
1
3
annotation2000134
1
N terminal 6 his
range2000134
1
1
27
annotation2000133
1
gly-ser
range2000133
1
22
27
BBa_J70034
1
BBa_J70034
A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)
2008-12-27T12:00:00Z
2015-05-08T01:08:20Z
Designed
When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar
still in testing, uses AarI (2) XmaI (1)
See part design page or BBFRFC15, for details about use
false
false
_41_
0
1201
41
Not in stock
false
TBA
false
Julie Norville
annotation2000048
1
AarI
range2000048
1
38
44
annotation2000047
1
AarI
range2000047
1
1
7
annotation2000049
1
XmaI
range2000049
1
20
25
BBa_J70048
1
BBa_J70048
6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA
2009-02-05T12:00:00Z
2015-05-08T01:08:20Z
Designed.
This 6 His suffix tag can be used with BBa_J70012 (PsrI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.
false
false
_41_
0
1201
41
Not in stock
false
Designed.
false
Julie Norville
annotation2000262
1
Start
range2000262
1
1
3
annotation2000266
1
stop
range2000266
1
28
30
annotation2000264
1
6 his
range2000264
1
10
27
annotation2000265
1
gly-ser
range2000265
1
4
9
annotation2000263
1
C terminal his tag
range2000263
1
1
30
BBa_J70110
1
BBa_J70110
Library Vector Test Plasmid for J70034
2009-02-06T12:00:00Z
2015-05-08T01:08:20Z
Designed
This is used to test whether J70034 (AarI, XmaI) can be used to make screening vectors.
false
false
_41_
0
1201
41
Not in stock
false
Where AarI digests
false
Julie Norville
component2000483
1
BBa_J70048
component2000473
1
BBa_J70042
component2000477
1
BBa_J70034
annotation2000483
1
BBa_J70048
range2000483
1
87
116
annotation2000477
1
BBa_J70034
range2000477
1
36
80
annotation2000473
1
BBa_J70042
range2000473
1
1
27
BBa_J70034_sequence
1
gcaggtggacaagaggagtcccgggagctggaactcccacctgca
BBa_J70110_sequence
1
atgcaccatcaccatcatcatggatcctactagaggcaggtggacaagaggagtcccgggagctggaactcccacctgcatactagatgggatcccaccatcaccatcatcattaa
BBa_J70042_sequence
1
atgcaccatcaccatcatcatggatcc
BBa_J70048_sequence
1
atgggatcccaccatcaccatcatcattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z