BBa_J70050
1
BBa_J70050
Left Library Insert Preparation Part (To use with J70052, and J70040)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
Still in Testing
Surround a part with J70050 and J70052, digest with AarI
Insert into a vector that contained J70040, and had J70040 removed by AarI and MabI
false
false
_41_
0
1201
41
Not in stock
false
Not sure if single AarI site will be good enough
false
Julie Norville
annotation2000145
1
Spacer
range2000145
1
8
11
annotation2000144
1
AarI
range2000144
1
1
7
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J70120
1
BBa_J70120
Library Insert Test Plasmid for J70050 and J70052
2009-02-06T12:00:00Z
2015-05-08T01:08:20Z
Designed
This is used to test whether J70050 and J70052 (AarI, MabI) can be used to make insert for screening vectors.
false
false
_41_
0
1201
41
Not in stock
false
Location of AarI sites
false
Julie Norville
component2000492
1
BBa_J70052
component2000486
1
BBa_J70050
component2000488
1
BBa_B0030
annotation2000492
1
BBa_J70052
range2000492
1
43
52
annotation2000486
1
BBa_J70050
range2000486
1
1
11
annotation2000488
1
BBa_B0030
range2000488
1
20
34
BBa_J70052
1
BBa_J70052
Right Library Insert Preparation Part (To use with J70050, and J70040)
2009-01-22T12:00:00Z
2015-05-08T01:08:20Z
Designed
Still in Testing
Surround a part with J70050 and J70052, digest with AarI
Insert into a vector that contained J70040 and had J70040 removed by AarI and MabI
false
false
_41_
0
1201
41
Not in stock
false
Not sure if one AarI site on each site will be enough.
false
Julie Norville
annotation2000146
1
Spacer
range2000146
1
1
3
annotation2000147
1
AarI
range2000147
1
4
10
BBa_J70050_sequence
1
cacctgcatat
BBa_J70052_sequence
1
atagcaggtg
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_J70120_sequence
1
cacctgcatattactagagattaaagaggagaaatactagagatagcaggtg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z