LOCUS       BBa_J70121              1034 bp    DNA     linear       22-Dec-2024
DEFINITION  R0040 regulated expression of a lacZa.GFP fusion protein
ACCESSION   BBa_J70121
VERSION     BBa_J70121.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..260
                     /label=lacZa
     misc_feature    261..317
                     /label=linker
     CDS             318..1034
                     /label=GFP
     misc_feature    81..1034
                     /label=BBa_E0051
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc gttttacaac
      121 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      181 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      241 gcctgaatgg cgaatggcgc gcgggcggca gcgaaggcgg cggcagcgaa catcatcatc
      301 atcatcatgg cagcgaacgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      361 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      421 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      481 catggccaac acttgtcact accctgacct atggtgttca atgctttgcg agatacccag
      541 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      601 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      661 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      721 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      781 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      841 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      901 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      961 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1021 tatacaaata ataa
//