LOCUS BBa_J70302 3980 bp DNA linear 26-Nov-2024 DEFINITION Test plasmid for Mesoplasma florum ACCESSION BBa_J70302 VERSION BBa_J70302.1 FEATURES Location/Qualifiers primer_bind 1..25 /label=J70023-F2 misc_feature 152..160 /label=dnaA binding misc_feature 218..226 /label=dnaA binding misc_feature 282..293 /label=imperfect repeat misc_feature 296..304 /label=dnaA binding misc_feature 403..413 /label=imperfect repeat misc_feature 587..595 /label=dnaA binding CDS 654..1307 /label=PE RBS 640..644 /label=RBS misc_feature 1305..1315 /label=iteron misc_feature 1325..1335 /label=iteron misc_feature 1470..1646 /label=Low GC region primer_bind 1678..1701 /label=J70023-R misc_feature 1..1701 /label=BBa_J70027 RBS 2048..2054 /label=RBS stem_loop 2028..2039 /label=term stem_loop 1982..2029 /label=stem_loop stem_loop 1963..1990 /label=stem_loop stem_loop 1951..1981 /label=stem_loop RBS 1946..1949 /label=rbs promoter 1777..1782 /label=-10 promoter 1754..1759 /label=-35 misc_feature 1789..1789 /label=+1 CDS 1959..2045 /label=leader peptide promoter 1710..2054 /label=BBa_J70025 CDS 2061..3980 /label=tetM gene CDS 2061..3980 /label=BBa_J70024 ORIGIN 1 taattataaa aacaccttat tttactaagg tgttttattt tttaacaaaa tatttgataa 61 atttatttat ctgttataca atgtaattgt tagcaattaa attgctagga caattaaaaa 121 cggaaacaca atgcaatttg aagacaactg tgtttccata aacatcttaa catattttat 181 ttaacttaga atgtgttata tcaattatta taatgtttgt ggaaatagcg gtctgttatt 241 tcccttttta tttgttacaa aaaaggagtt gaaattaaat tatttaatcc ccctttgtgg 301 tagaataaaa acaactaaat agaaacaaag aaaaatgact cccacgtcat tcttctatac 361 acctattaag cggtctcaca aaccacttaa ctagggttct taatttaacc cccacctttt 421 ttggtaggtc agttaaatta taacattttt taatttttgt gtataggtag agtgataaaa 481 aagtcattct acctattttt tttatttaat atatcaaaac aacaaaaaaa tcagtttata 541 tcagcgttga aataaccgaa aagaatgaag agaagattta ttacattttg gaaaatatca 601 acgctgatat ggggtttaaa ttcgctcaaa accgtagaaa ggagcatatt attatgcaac 661 atcaacaaca aacagaacaa ctcaattatc actttgttga gataaatggg ttgagttata 721 aagtgattaa tcaacttgac gagaaaaaac atatctctaa tttttactta ccgaaaaaaa 781 ttgttcgcca acaccctacc cgccaagaca gttataaagt caaaatttat gacaaattta 841 tttgtgttcc taaaaaaatg tgtttcttag acaaaactgg taaatacttt ttggttggtc 901 ttgatatgtt ttttaactac tgaatttata atacacgaga taataacaaa tatcgcttga 961 cgggttatca agcgattaaa gatactgcaa ttaaagaatt gcattattta actgtgtctg 1021 ctcgccgcta cgaaaaagaa caagcaacaa accctttttt aagtggatta acttaccaac 1081 aagcacgcga ccaaatttgt gctgaaagcg acaaactaaa agcagaatac caatctttcg 1141 taaaaactaa gtattctgct aaaaacagtg aaaatttact taccagagaa aataattctc 1201 ttgttgatga ttataacact gtttatgaaa aaaacaaggg agaaccacca gaaatgtccg 1261 aagaagaact cactgagtta ttaacggaat tagacaactt agactaactc ccctatcgag 1321 ttcttaactc ccctaccaac aaagcacaga acttgcgtaa aaccacagcg tagcaagaca 1381 atgaatataa tactaagata tggtctttat catatcgttt ttgctgttta gtaaaaagcg 1441 atatttttta tttctttttc tcccaataga aaaccaaaat aaataccatt actatatcaa 1501 aacaaaatat ttaataaatt ttatatactt ttttaagcta ctatttagat tattttattt 1561 aaaaagtact aaatatatta aaatttaaga tataatttat atagtaatat aatagagaat 1621 aataagtatt tacatttttt attatactgc ggaacaaaaa gcattttggc acgccaagca 1681 ccccaaaccc cgcactccca atactagagg gccagtctac atgtactctt tttgataaaa 1741 aattggagat tcctttacaa atatgctctt acgtgctatt atttaagtga ctatttaaaa 1801 ggagttaata aatatgcggc aaggtattct taaataaact gtcaatttga tagcgggaac 1861 aaataattag atgtcctttt ttaggagggc ttagtttttt gtacccagtt taagaatacc 1921 tttatcatgt gattctaaag tatccagaga atatctgtat gctttgtata cctatggtta 1981 tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc cttttgggtt 2041 tttgaatgga ggaatactag atgaaaatca taaatatcgg tgtattagct cacgttgatg 2101 caggaaaaac aacattaact gaatcacttt tatataactc tggtgcaatt actgaacttg 2161 gttcagtaga taaaggtact actcgtactg ataatacatt attagaacgt caacgtggaa 2221 tcacaattca aacaggtatc acatcttttc aatgggaaaa tacaaaagta aatattatag 2281 atacacctgg acacatggat ttccttgcag aagtataccg tagtctttca gtattagatg 2341 gtgctatttt acttatcagc gctaaagatg gagttcaagc tcaaactcgt atcttatttc 2401 acgcattacg taaaatgggt attccaacaa ttttctttat aaacaaaatt gaccaaaacg 2461 gaattgattt aagtacagtt tatcaagata tcaaagaaaa actttctgct gaaatcgtta 2521 ttaaacaaaa agttgaatta tacccaaacg tttgcgtaac aaattttact gaatcagaac 2581 aatgggatac agttatagaa ggtaatgatg atttattaga aaaatacatg tcaggtaaat 2641 cattagaagc attagaatta gaacaagaag aaagtattcg tttccaaaac tgttctttat 2701 tccctttata ccatggaagc gctaaaagta acataggtat tgataactta attgaagtta 2761 ttactaacaa attttactct tcaactcatc gtggtccttc tgaattatgc ggtaacgttt 2821 tcaaaattga atatacaaaa aaacgtcaac gtttagctta tatacgtctt tatagtggtg 2881 ttttacattt acgtgatagt gttcgtgtta gtgaaaaaga aaagattaaa gttacagaaa 2941 tgtatacttc tattaacggt gaattatgca aaattgaccg tgcatattca ggtgaaattg 3001 taattttaca aaacgaattt cttaaactta atagtgtact tggtgacaca aaacttttac 3061 cacaacgtaa gaaaattgaa aatccacacc cattacttca aacaacagta gaaccaagca 3121 aacctgaaca acgtgaaatg cttttagatg ctcttttaga aattagtgac tctgacccac 3181 ttttacgtta ctatgtagat tctactactc atgaaattat tctttctttc cttggtaaag 3241 ttcaaatgga agttatttct gcattattac aagaaaaata tcatgttgaa atcgaattaa 3301 aagaacctac tgtaatttat atggaacgtc cattaaaaaa tgctgaatat acaattcata 3361 ttgaagttcc accaaatcca ttttgggctt ctattggtct ttctgtttct ccacttccac 3421 ttggtagcgg aatgcaatat gaaagtagcg taagtttagg ttatcttaat caaagtttcc 3481 aaaacgcagt tatggaaggt attcgttacg gttgcgaaca aggtttatac ggttggaatg 3541 ttacagactg caaaatctgt tttaagtatg gactttacta ttcacctgta tcaacacctg 3601 ctgactttcg tatgcttgca ccaattgttt tagaacaagt tttaaagaaa gctggaactg 3661 aacttttaga accatacctt tcttttaaaa tctatgcacc acaagaatac ttaagtcgtg 3721 cttataacga tgcacctaaa tactgtgcta atattgttga tactcaatta aagaacaacg 3781 aagtaatttt aagcggagaa attcctgcac gttgtattca agaatatcgt agtgatttaa 3841 catttttcac taatggacgt tctgtttgct taactgaatt aaaaggttat catgttacta 3901 ctggtgaacc tgtatgccaa ccacgtcgtc ctaatagtcg tattgataaa gttcgttata 3961 tgttcaacaa aatcacataa //