BBa_J70302 1 BBa_J70302 Test plasmid for Mesoplasma florum 2009-04-17T11:00:00Z 2015-05-08T01:08:20Z Assembly from two registry parts. Assembly of tetM cassette and pSCI2 plasmid origin into E. coli plasmid pSB1K3. false false _41_48_1_ 0 6 48 Not in stock false Assembled into Kan resistant part from cm resistant parts. Avoid tet resistant backbone plasmids, since the insert is tet resistant. false Tom Knight component2002424 1 BBa_J70024 component2002411 1 BBa_J70027 component2002422 1 BBa_J70025 annotation2002411 1 BBa_J70027 range2002411 1 1 1701 annotation2002424 1 BBa_J70024 range2002424 1 2061 3980 annotation2002422 1 BBa_J70025 range2002422 1 1710 2054 BBa_J70027 1 BBa_J70027 Mesoplasma florum plasmid origin 2008-11-04T12:00:00Z 2015-05-08T01:08:20Z Genbank AJ969070 This part is a cassette containing the PE gene from the pSci2 plasmid of Spiroplasma citri. It is reported to convey replication ability for the group 1 spiroplasmas. The part contains the replication origin, the promoter, and gene for the PE protein. false false _41_48_1_ 0 6 48 Not in stock false No native restriction sites. Added biobrick ends. The gene for PE contains no CGG codons. GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2 GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R The start of J70027 matches the origin start in the pSci1 plasmid. false Tom Knight annotation1999316 1 imperfect repeat range1999316 1 403 413 annotation1999319 1 RBS range1999319 1 640 644 annotation1999338 1 Low GC region range1999338 1 1470 1646 annotation1999315 1 dnaA binding range1999315 1 296 304 annotation1999313 1 dnaA binding range1999313 1 218 226 annotation1999318 1 PE range1999318 1 654 1307 annotation1999311 1 J70023-F2 range1999311 1 1 25 annotation1999339 1 J70023-R range1999339 1 1678 1701 annotation1999312 1 dnaA binding range1999312 1 152 160 annotation1999320 1 iteron range1999320 1 1305 1315 annotation1999321 1 iteron range1999321 1 1325 1335 annotation1999317 1 dnaA binding range1999317 1 587 595 annotation1999314 1 imperfect repeat range1999314 1 282 293 BBa_J70024 1 BBa_J70024 TetM gene, recoded compromise E. coli & Mesoplasma florum 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z Synthesized from the Ureaplasma urealyticum tetM gene sequence, with recoding. The tetM antibiotic resistance gene synthesized by Geneart from a protein sequence derived from the Ureaplasma urealyticum tetM gene. Synthesized with a compromise codon usage table for expression in both E. coli and Mesoplasma florum. Highly active in both E. coli and Mesoplasma florum. false false _41_48_1_ 0 6 48 Not in stock true Elimination of restriction sites, CGG codons, UGA codons, rare codons in both species. false Tom Knight annotation1982961 1 tetM gene range1982961 1 1 1920 BBa_J70025 1 BBa_J70025 Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid. Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024. false false _41_48_1_ 0 6 48 Not in stock true Alignment of the promoter with the coding region, preservation of the RBS sites. false Tom Knight annotation1999384 1 stem_loop range1999384 1 273 320 annotation1999388 1 -10 range1999388 1 68 73 annotation1999390 1 +1 range1999390 1 80 80 annotation1999385 1 stem_loop range1999385 1 254 281 annotation1999387 1 rbs range1999387 1 237 240 annotation1999382 1 RBS range1999382 1 339 345 annotation1999383 1 term range1999383 1 319 330 annotation1999386 1 stem_loop range1999386 1 242 272 annotation1999391 1 leader peptide range1999391 1 250 336 annotation1999389 1 -35 range1999389 1 45 50 BBa_J70024_sequence 1 atgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_J70302_sequence 1 taattataaaaacaccttattttactaaggtgttttattttttaacaaaatatttgataaatttatttatctgttatacaatgtaattgttagcaattaaattgctaggacaattaaaaacggaaacacaatgcaatttgaagacaactgtgtttccataaacatcttaacatattttatttaacttagaatgtgttatatcaattattataatgtttgtggaaatagcggtctgttatttccctttttatttgttacaaaaaaggagttgaaattaaattatttaatccccctttgtggtagaataaaaacaactaaatagaaacaaagaaaaatgactcccacgtcattcttctatacacctattaagcggtctcacaaaccacttaactagggttcttaatttaacccccaccttttttggtaggtcagttaaattataacattttttaatttttgtgtataggtagagtgataaaaaagtcattctacctattttttttatttaatatatcaaaacaacaaaaaaatcagtttatatcagcgttgaaataaccgaaaagaatgaagagaagatttattacattttggaaaatatcaacgctgatatggggtttaaattcgctcaaaaccgtagaaaggagcatattattatgcaacatcaacaacaaacagaacaactcaattatcactttgttgagataaatgggttgagttataaagtgattaatcaacttgacgagaaaaaacatatctctaatttttacttaccgaaaaaaattgttcgccaacaccctacccgccaagacagttataaagtcaaaatttatgacaaatttatttgtgttcctaaaaaaatgtgtttcttagacaaaactggtaaatactttttggttggtcttgatatgttttttaactactgaatttataatacacgagataataacaaatatcgcttgacgggttatcaagcgattaaagatactgcaattaaagaattgcattatttaactgtgtctgctcgccgctacgaaaaagaacaagcaacaaacccttttttaagtggattaacttaccaacaagcacgcgaccaaatttgtgctgaaagcgacaaactaaaagcagaataccaatctttcgtaaaaactaagtattctgctaaaaacagtgaaaatttacttaccagagaaaataattctcttgttgatgattataacactgtttatgaaaaaaacaagggagaaccaccagaaatgtccgaagaagaactcactgagttattaacggaattagacaacttagactaactcccctatcgagttcttaactcccctaccaacaaagcacagaacttgcgtaaaaccacagcgtagcaagacaatgaatataatactaagatatggtctttatcatatcgtttttgctgtttagtaaaaagcgatattttttatttctttttctcccaatagaaaaccaaaataaataccattactatatcaaaacaaaatatttaataaattttatatacttttttaagctactatttagattattttatttaaaaagtactaaatatattaaaatttaagatataatttatatagtaatataatagagaataataagtatttacattttttattatactgcggaacaaaaagcattttggcacgccaagcaccccaaaccccgcactcccaatactagagggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaatactagatgaaaatcataaatatcggtgtattagctcacgttgatgcaggaaaaacaacattaactgaatcacttttatataactctggtgcaattactgaacttggttcagtagataaaggtactactcgtactgataatacattattagaacgtcaacgtggaatcacaattcaaacaggtatcacatcttttcaatgggaaaatacaaaagtaaatattatagatacacctggacacatggatttccttgcagaagtataccgtagtctttcagtattagatggtgctattttacttatcagcgctaaagatggagttcaagctcaaactcgtatcttatttcacgcattacgtaaaatgggtattccaacaattttctttataaacaaaattgaccaaaacggaattgatttaagtacagtttatcaagatatcaaagaaaaactttctgctgaaatcgttattaaacaaaaagttgaattatacccaaacgtttgcgtaacaaattttactgaatcagaacaatgggatacagttatagaaggtaatgatgatttattagaaaaatacatgtcaggtaaatcattagaagcattagaattagaacaagaagaaagtattcgtttccaaaactgttctttattccctttataccatggaagcgctaaaagtaacataggtattgataacttaattgaagttattactaacaaattttactcttcaactcatcgtggtccttctgaattatgcggtaacgttttcaaaattgaatatacaaaaaaacgtcaacgtttagcttatatacgtctttatagtggtgttttacatttacgtgatagtgttcgtgttagtgaaaaagaaaagattaaagttacagaaatgtatacttctattaacggtgaattatgcaaaattgaccgtgcatattcaggtgaaattgtaattttacaaaacgaatttcttaaacttaatagtgtacttggtgacacaaaacttttaccacaacgtaagaaaattgaaaatccacacccattacttcaaacaacagtagaaccaagcaaacctgaacaacgtgaaatgcttttagatgctcttttagaaattagtgactctgacccacttttacgttactatgtagattctactactcatgaaattattctttctttccttggtaaagttcaaatggaagttatttctgcattattacaagaaaaatatcatgttgaaatcgaattaaaagaacctactgtaatttatatggaacgtccattaaaaaatgctgaatatacaattcatattgaagttccaccaaatccattttgggcttctattggtctttctgtttctccacttccacttggtagcggaatgcaatatgaaagtagcgtaagtttaggttatcttaatcaaagtttccaaaacgcagttatggaaggtattcgttacggttgcgaacaaggtttatacggttggaatgttacagactgcaaaatctgttttaagtatggactttactattcacctgtatcaacacctgctgactttcgtatgcttgcaccaattgttttagaacaagttttaaagaaagctggaactgaacttttagaaccatacctttcttttaaaatctatgcaccacaagaatacttaagtcgtgcttataacgatgcacctaaatactgtgctaatattgttgatactcaattaaagaacaacgaagtaattttaagcggagaaattcctgcacgttgtattcaagaatatcgtagtgatttaacatttttcactaatggacgttctgtttgcttaactgaattaaaaggttatcatgttactactggtgaacctgtatgccaaccacgtcgtcctaatagtcgtattgataaagttcgttatatgttcaacaaaatcacataa BBa_J70025_sequence 1 ggccagtctacatgtactctttttgataaaaaattggagattcctttacaaatatgctcttacgtgctattatttaagtgactatttaaaaggagttaataaatatgcggcaaggtattcttaaataaactgtcaatttgatagcgggaacaaataattagatgtccttttttaggagggcttagttttttgtacccagtttaagaatacctttatcatgtgattctaaagtatccagagaatatctgtatgctttgtatacctatggttatgcataaaaatcccagtgataaaagtatttatcactgggatttttatgcccttttgggtttttgaatggaggaa BBa_J70027_sequence 1 taattataaaaacaccttattttactaaggtgttttattttttaacaaaatatttgataaatttatttatctgttatacaatgtaattgttagcaattaaattgctaggacaattaaaaacggaaacacaatgcaatttgaagacaactgtgtttccataaacatcttaacatattttatttaacttagaatgtgttatatcaattattataatgtttgtggaaatagcggtctgttatttccctttttatttgttacaaaaaaggagttgaaattaaattatttaatccccctttgtggtagaataaaaacaactaaatagaaacaaagaaaaatgactcccacgtcattcttctatacacctattaagcggtctcacaaaccacttaactagggttcttaatttaacccccaccttttttggtaggtcagttaaattataacattttttaatttttgtgtataggtagagtgataaaaaagtcattctacctattttttttatttaatatatcaaaacaacaaaaaaatcagtttatatcagcgttgaaataaccgaaaagaatgaagagaagatttattacattttggaaaatatcaacgctgatatggggtttaaattcgctcaaaaccgtagaaaggagcatattattatgcaacatcaacaacaaacagaacaactcaattatcactttgttgagataaatgggttgagttataaagtgattaatcaacttgacgagaaaaaacatatctctaatttttacttaccgaaaaaaattgttcgccaacaccctacccgccaagacagttataaagtcaaaatttatgacaaatttatttgtgttcctaaaaaaatgtgtttcttagacaaaactggtaaatactttttggttggtcttgatatgttttttaactactgaatttataatacacgagataataacaaatatcgcttgacgggttatcaagcgattaaagatactgcaattaaagaattgcattatttaactgtgtctgctcgccgctacgaaaaagaacaagcaacaaacccttttttaagtggattaacttaccaacaagcacgcgaccaaatttgtgctgaaagcgacaaactaaaagcagaataccaatctttcgtaaaaactaagtattctgctaaaaacagtgaaaatttacttaccagagaaaataattctcttgttgatgattataacactgtttatgaaaaaaacaagggagaaccaccagaaatgtccgaagaagaactcactgagttattaacggaattagacaacttagactaactcccctatcgagttcttaactcccctaccaacaaagcacagaacttgcgtaaaaccacagcgtagcaagacaatgaatataatactaagatatggtctttatcatatcgtttttgctgtttagtaaaaagcgatattttttatttctttttctcccaatagaaaaccaaaataaataccattactatatcaaaacaaaatatttaataaattttatatacttttttaagctactatttagattattttatttaaaaagtactaaatatattaaaatttaagatataatttatatagtaatataatagagaataataagtatttacattttttattatactgcggaacaaaaagcattttggcacgccaagcaccccaaaccccgcactcccaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z