LOCUS BBa_J70303 2739 bp DNA linear 23-Dec-2024 DEFINITION Test Cm resistance plasmid for Mesoplasma florum ACCESSION BBa_J70303 VERSION BBa_J70303.1 FEATURES Location/Qualifiers primer_bind 1..25 /label=J70023-F2 misc_feature 152..160 /label=dnaA binding misc_feature 218..226 /label=dnaA binding misc_feature 282..293 /label=imperfect repeat misc_feature 296..304 /label=dnaA binding misc_feature 403..413 /label=imperfect repeat misc_feature 587..595 /label=dnaA binding CDS 654..1307 /label=PE RBS 640..644 /label=RBS misc_feature 1305..1315 /label=iteron misc_feature 1325..1335 /label=iteron misc_feature 1470..1646 /label=Low GC region primer_bind 1678..1701 /label=J70023-R misc_feature 1..1701 /label=BBa_J70027 misc_feature 1866..1888 /label=UP sequence misc_feature 1901..1906 /label=-35 misc_feature 1924..1929 /label=-10 RBS 1980..1984 /label=RBS CDS 1993..2643 /label=cat gene stem_loop 2655..2678 /label=terminator misc_feature 1710..2739 /label=BBa_J70026 ORIGIN 1 taattataaa aacaccttat tttactaagg tgttttattt tttaacaaaa tatttgataa 61 atttatttat ctgttataca atgtaattgt tagcaattaa attgctagga caattaaaaa 121 cggaaacaca atgcaatttg aagacaactg tgtttccata aacatcttaa catattttat 181 ttaacttaga atgtgttata tcaattatta taatgtttgt ggaaatagcg gtctgttatt 241 tcccttttta tttgttacaa aaaaggagtt gaaattaaat tatttaatcc ccctttgtgg 301 tagaataaaa acaactaaat agaaacaaag aaaaatgact cccacgtcat tcttctatac 361 acctattaag cggtctcaca aaccacttaa ctagggttct taatttaacc cccacctttt 421 ttggtaggtc agttaaatta taacattttt taatttttgt gtataggtag agtgataaaa 481 aagtcattct acctattttt tttatttaat atatcaaaac aacaaaaaaa tcagtttata 541 tcagcgttga aataaccgaa aagaatgaag agaagattta ttacattttg gaaaatatca 601 acgctgatat ggggtttaaa ttcgctcaaa accgtagaaa ggagcatatt attatgcaac 661 atcaacaaca aacagaacaa ctcaattatc actttgttga gataaatggg ttgagttata 721 aagtgattaa tcaacttgac gagaaaaaac atatctctaa tttttactta ccgaaaaaaa 781 ttgttcgcca acaccctacc cgccaagaca gttataaagt caaaatttat gacaaattta 841 tttgtgttcc taaaaaaatg tgtttcttag acaaaactgg taaatacttt ttggttggtc 901 ttgatatgtt ttttaactac tgaatttata atacacgaga taataacaaa tatcgcttga 961 cgggttatca agcgattaaa gatactgcaa ttaaagaatt gcattattta actgtgtctg 1021 ctcgccgcta cgaaaaagaa caagcaacaa accctttttt aagtggatta acttaccaac 1081 aagcacgcga ccaaatttgt gctgaaagcg acaaactaaa agcagaatac caatctttcg 1141 taaaaactaa gtattctgct aaaaacagtg aaaatttact taccagagaa aataattctc 1201 ttgttgatga ttataacact gtttatgaaa aaaacaaggg agaaccacca gaaatgtccg 1261 aagaagaact cactgagtta ttaacggaat tagacaactt agactaactc ccctatcgag 1321 ttcttaactc ccctaccaac aaagcacaga acttgcgtaa aaccacagcg tagcaagaca 1381 atgaatataa tactaagata tggtctttat catatcgttt ttgctgttta gtaaaaagcg 1441 atatttttta tttctttttc tcccaataga aaaccaaaat aaataccatt actatatcaa 1501 aacaaaatat ttaataaatt ttatatactt ttttaagcta ctatttagat tattttattt 1561 aaaaagtact aaatatatta aaatttaaga tataatttat atagtaatat aatagagaat 1621 aataagtatt tacatttttt attatactgc ggaacaaaaa gcattttggc acgccaagca 1681 ccccaaaccc cgcactccca atactagaga atagttaccc ttattatcaa gataagaaag 1741 aaaaggattt ttcgctacgc tcaaatcctt taaaaaaaca caaaagacca cattttttaa 1801 tgtggtcttt tattcttcaa ctaaagcacc cattagttca acaaacgaaa attggataaa 1861 gtgggatatt tttaaaatat atatttatgt tacagtaata ttgactttta aaaaaggatt 1921 gattctaatg aagaaagcag acaagtaagc ctcctaaatt cactttagat aaaaatttag 1981 gaggcatatc aaatgaactt taataaaatt gatttagaca attggaagag aaaagagata 2041 tttaatcatt atttgaacca acaaacgact tttagtataa ccacagaaat tgatattagt 2101 gttttatacc gaaacataaa acaagaagga tataaatttt accctgcatt tattttctta 2161 gtgacaaggg tgataaactc aaatacagct tttagaactg gttacaatag cgacggagag 2221 ttaggttatt gggataagtt agagccactt tatacaattt ttgatggtgt atctaaaaca 2281 ttctctggta tttggactcc tgtaaagaat gacttcaaag agttttatga tttatacctt 2341 tctgatgtag agaaatataa tggttcgggg aaattgtttc ccaaaacacc tatacctgaa 2401 aatgcttttt ctctttctat tattccatgg acttcattta ctgggtttaa cttaaatatc 2461 aataataata gtaattacct tctacccatt attacagcag gaaaattcat taataaaggt 2521 aattcaatat atttaccgct atctttacag gtacatcatt ctgtttgtga tggttatcat 2581 gcaggattgt ttatgaactc tattcaggaa ttgtcagata ggcctaatga ctggctttta 2641 taatatgaga taatgccgac tgtacttttt acagtcggtt ttctaatgtc actaacctgc 2701 cccgttagtt gaagaaggtt tttatattac agctccaga //