BBa_J70303 1 BBa_J70303 Test Cm resistance plasmid for Mesoplasma florum 2009-04-19T11:00:00Z 2015-05-08T01:08:20Z Standard assembly from registry parts. Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3. false false _41_48_1_ 0 6 48 Not in stock false Avoid assembly into a Cm resistant plasmid, as the construct is already Cm resistant. false Tom Knight component2002523 1 BBa_J70026 component2002517 1 BBa_J70027 annotation2002517 1 BBa_J70027 range2002517 1 1 1701 annotation2002523 1 BBa_J70026 range2002523 1 1710 2739 BBa_J70026 1 BBa_J70026 cat cassette (chloramphenicol resistance) 2008-10-19T11:00:00Z 2015-05-08T01:08:20Z Genbank V01277, by way of pKV104 from Duncan Krause. Bases 978-2006; missing T in the Krause sequence at base 99 of this sequence. cat gene and promoter from pC194 plasmid, Genbank V01277. false false _41_48_1_ 0 6 48 Not in stock false Mutation in sequence at base 99, ignored. GATC site eliminated at the end by truncation. false Tom Knight annotation2002628 1 UP sequence range2002628 1 157 179 annotation2002631 1 RBS range2002631 1 271 275 annotation2002629 1 -35 range2002629 1 192 197 annotation2002630 1 -10 range2002630 1 215 220 annotation2002633 1 terminator range2002633 1 946 969 annotation2002632 1 cat gene range2002632 1 284 934 BBa_J70027 1 BBa_J70027 Mesoplasma florum plasmid origin 2008-11-04T12:00:00Z 2015-05-08T01:08:20Z Genbank AJ969070 This part is a cassette containing the PE gene from the pSci2 plasmid of Spiroplasma citri. It is reported to convey replication ability for the group 1 spiroplasmas. The part contains the replication origin, the promoter, and gene for the PE protein. false false _41_48_1_ 0 6 48 Not in stock false No native restriction sites. Added biobrick ends. The gene for PE contains no CGG codons. GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2 GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R The start of J70027 matches the origin start in the pSci1 plasmid. false Tom Knight annotation1999311 1 J70023-F2 range1999311 1 1 25 annotation1999312 1 dnaA binding range1999312 1 152 160 annotation1999314 1 imperfect repeat range1999314 1 282 293 annotation1999313 1 dnaA binding range1999313 1 218 226 annotation1999318 1 PE range1999318 1 654 1307 annotation1999338 1 Low GC region range1999338 1 1470 1646 annotation1999317 1 dnaA binding range1999317 1 587 595 annotation1999319 1 RBS range1999319 1 640 644 annotation1999339 1 J70023-R range1999339 1 1678 1701 annotation1999320 1 iteron range1999320 1 1305 1315 annotation1999316 1 imperfect repeat range1999316 1 403 413 annotation1999315 1 dnaA binding range1999315 1 296 304 annotation1999321 1 iteron range1999321 1 1325 1335 BBa_J70026_sequence 1 aatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtcttttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccaga BBa_J70027_sequence 1 taattataaaaacaccttattttactaaggtgttttattttttaacaaaatatttgataaatttatttatctgttatacaatgtaattgttagcaattaaattgctaggacaattaaaaacggaaacacaatgcaatttgaagacaactgtgtttccataaacatcttaacatattttatttaacttagaatgtgttatatcaattattataatgtttgtggaaatagcggtctgttatttccctttttatttgttacaaaaaaggagttgaaattaaattatttaatccccctttgtggtagaataaaaacaactaaatagaaacaaagaaaaatgactcccacgtcattcttctatacacctattaagcggtctcacaaaccacttaactagggttcttaatttaacccccaccttttttggtaggtcagttaaattataacattttttaatttttgtgtataggtagagtgataaaaaagtcattctacctattttttttatttaatatatcaaaacaacaaaaaaatcagtttatatcagcgttgaaataaccgaaaagaatgaagagaagatttattacattttggaaaatatcaacgctgatatggggtttaaattcgctcaaaaccgtagaaaggagcatattattatgcaacatcaacaacaaacagaacaactcaattatcactttgttgagataaatgggttgagttataaagtgattaatcaacttgacgagaaaaaacatatctctaatttttacttaccgaaaaaaattgttcgccaacaccctacccgccaagacagttataaagtcaaaatttatgacaaatttatttgtgttcctaaaaaaatgtgtttcttagacaaaactggtaaatactttttggttggtcttgatatgttttttaactactgaatttataatacacgagataataacaaatatcgcttgacgggttatcaagcgattaaagatactgcaattaaagaattgcattatttaactgtgtctgctcgccgctacgaaaaagaacaagcaacaaacccttttttaagtggattaacttaccaacaagcacgcgaccaaatttgtgctgaaagcgacaaactaaaagcagaataccaatctttcgtaaaaactaagtattctgctaaaaacagtgaaaatttacttaccagagaaaataattctcttgttgatgattataacactgtttatgaaaaaaacaagggagaaccaccagaaatgtccgaagaagaactcactgagttattaacggaattagacaacttagactaactcccctatcgagttcttaactcccctaccaacaaagcacagaacttgcgtaaaaccacagcgtagcaagacaatgaatataatactaagatatggtctttatcatatcgtttttgctgtttagtaaaaagcgatattttttatttctttttctcccaatagaaaaccaaaataaataccattactatatcaaaacaaaatatttaataaattttatatacttttttaagctactatttagattattttatttaaaaagtactaaatatattaaaatttaagatataatttatatagtaatataatagagaataataagtatttacattttttattatactgcggaacaaaaagcattttggcacgccaagcaccccaaaccccgcactcccaa BBa_J70303_sequence 1 taattataaaaacaccttattttactaaggtgttttattttttaacaaaatatttgataaatttatttatctgttatacaatgtaattgttagcaattaaattgctaggacaattaaaaacggaaacacaatgcaatttgaagacaactgtgtttccataaacatcttaacatattttatttaacttagaatgtgttatatcaattattataatgtttgtggaaatagcggtctgttatttccctttttatttgttacaaaaaaggagttgaaattaaattatttaatccccctttgtggtagaataaaaacaactaaatagaaacaaagaaaaatgactcccacgtcattcttctatacacctattaagcggtctcacaaaccacttaactagggttcttaatttaacccccaccttttttggtaggtcagttaaattataacattttttaatttttgtgtataggtagagtgataaaaaagtcattctacctattttttttatttaatatatcaaaacaacaaaaaaatcagtttatatcagcgttgaaataaccgaaaagaatgaagagaagatttattacattttggaaaatatcaacgctgatatggggtttaaattcgctcaaaaccgtagaaaggagcatattattatgcaacatcaacaacaaacagaacaactcaattatcactttgttgagataaatgggttgagttataaagtgattaatcaacttgacgagaaaaaacatatctctaatttttacttaccgaaaaaaattgttcgccaacaccctacccgccaagacagttataaagtcaaaatttatgacaaatttatttgtgttcctaaaaaaatgtgtttcttagacaaaactggtaaatactttttggttggtcttgatatgttttttaactactgaatttataatacacgagataataacaaatatcgcttgacgggttatcaagcgattaaagatactgcaattaaagaattgcattatttaactgtgtctgctcgccgctacgaaaaagaacaagcaacaaacccttttttaagtggattaacttaccaacaagcacgcgaccaaatttgtgctgaaagcgacaaactaaaagcagaataccaatctttcgtaaaaactaagtattctgctaaaaacagtgaaaatttacttaccagagaaaataattctcttgttgatgattataacactgtttatgaaaaaaacaagggagaaccaccagaaatgtccgaagaagaactcactgagttattaacggaattagacaacttagactaactcccctatcgagttcttaactcccctaccaacaaagcacagaacttgcgtaaaaccacagcgtagcaagacaatgaatataatactaagatatggtctttatcatatcgtttttgctgtttagtaaaaagcgatattttttatttctttttctcccaatagaaaaccaaaataaataccattactatatcaaaacaaaatatttaataaattttatatacttttttaagctactatttagattattttatttaaaaagtactaaatatattaaaatttaagatataatttatatagtaatataatagagaataataagtatttacattttttattatactgcggaacaaaaagcattttggcacgccaagcaccccaaaccccgcactcccaatactagagaatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtcttttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z