BBa_J70304 1 BBa_J70304 constitutive cat cassette for Mesoplasma florum 2009-05-05T11:00:00Z 2015-05-08T01:08:20Z PCR from pSB1T3-J70026 with primers GTTTCTTCggtacctttcttcattagaatcaatcc,cat-mut-R GTTTCTTCggtacctttaggaggcatatcaaatg,cat-mut-F These primers bind to the source plasmid and remove the left side of the hairpin and add a pair of KpnI sites, which will be cut and religated. I hope to select on Cm plates, since the construct should be Cm resistant. This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct. false false _41_48_1_ 0 6 48 Not in stock false Test for KpnI sites in the source plasmid. false Tom Knight annotation2002620 1 -35 range2002620 1 192 197 annotation2002619 1 UP sequence range2002619 1 157 179 annotation2002622 1 rbs range2002622 1 237 241 annotation2002623 1 cat range2002623 1 251 901 annotation2002624 1 terminator range2002624 1 914 936 annotation2002625 1 KpnI site range2002625 1 227 233 annotation2002621 1 -10 range2002621 1 215 220 BBa_J70304_sequence 1 aatagttacccttattatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtcttttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaaggtacctttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z